逐痰通络汤延缓大鼠骨关节炎软骨退变的实验研究

Zhutan Tongluo Tang ameliorated the degeneration of articular cartilage in a rat osteoarthritis model

目的研究逐痰通络汤干预SD大鼠膝骨关节炎的作用及机制。方法选取30只SD大鼠,分为健康对照组、模型对照组以及逐痰通络汤给药组,每组10只。利用4%木瓜蛋白酶诱导制作骨关节炎模型。造模后,逐痰通络汤给药组用2mL/(kg·d)逐痰通络汤灌胃,模型对照组以等量的生理盐水灌胃,健康对照组常规喂养。持续灌胃3个月后,采用HE染色法观察软骨形态结构的变化,酶联免疫吸附试验(ELISA)法检测关节液中白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α以及基质金属蛋白酶(MMP)-3水平的变化。结果逐痰通络汤给药组的软骨退变程度明显好于模型对照组。逐痰通络汤给药组大鼠关节液中的IL-1β(12.13pg/mL)、TNF-α(13.76pg/mL)和MMP-3(14.21ng/mL)水平较模型对照组(16.62pg/mL、18.26pg/mL、21.57ng/mL)显著降低,差异有统计学意义(P<0.05)。结论逐痰通络汤具有延缓骨关节炎软骨退变和减轻炎症的作用,其机制与抑制关节液中炎症因子的表达有关。

Objective To investigate the effect of Zhutan Tongluo Tang on the articular cartilage degeneration in a rat osteoarthritis model,and explore the potential mechanism.Methods Thirty-healthy SD rats were equally divided into the normal control group,the model control group and Zhutan Tongluo Tang group.Rats were induced osteoarthritis models with 4% mass fraction of papain.After replication of osteoarthritis models,Zhutan Tongluo Tang group were given Zhutan Tongluo Tang with 2 mL/（kg·d）,the model control group were given lavage with normal saline,the normal control group with rovtine feeding.After treatments for 3 months,the cartilage morphology were observed using HE stains,and the expression of IL-1β,TNF-αand MMP-3 in synovial fluid were measured by ELISA.Results The degeneration of cartilage in Zhutan Tongluo Tang group was better than the model control group.Compared with the model control group,the expression levels of IL-1β（12.13 pg/mL vs.16.62pg/mL）,TNF-α（13.76 pg/mL vs.18.26 pg/mL）and MMP-3（14.21 ng/mL vs.21.57 ng/mL）in synovial fluid were significantly decreased in Zhutan Tongluo Tang group（P〈0.05）.Conclusion Zhutan Tongluo Tang has the function of treating delaying osteoarthritis cartilage degeneration and decreasing inflammation,whose mechanism is related to inhibiting the expression of inflammatory cytokines.