iTRAQ多重标记串联质谱技术在乳腺癌细胞侵袭患者HGF表达中的应用

Application of iTRAQ multiplex tandem mass spectrometry in the expression of HGF in breast cancer cell invasion

目的探讨iTRAQ多重标记串联质谱技术应用于检测乳腺癌细胞侵袭患者肝细胞生长因子(HGF)表达差异的临床价值。方法选择该院2014年1月至2016年10月收治的35例乳腺癌患者与30例健康者为研究对象,通过iTRAQ标记、质谱检测、搜库以及Scqffold软件分析不同临床分期乳腺癌患者与健康者血清HGF表达差异,并用Western blot验证HGF的差异表达。结果该研究血清样品中共鉴定出蛋白237个,达到严格定量标准的蛋白89个,乳腺癌患者与健康者共筛选出包括HGF在内的差异表达蛋白17个;iTRAQ多重标记串联质谱图谱显示,乳腺癌患者血清HGF表达水平显著高于健康者,差异有统计学意义(P<0.05);Western blot结果显示,不同临床分期HGF相对表达水平显著高于健康者,差异有统计学意义(P<0.05)。结论iTRAQ多重标记串联质谱技术有助于发现乳腺癌患者癌细胞高表达HGF,对指导临床治疗乳腺癌具有重要意义。

Objective To investigate the clinical value of iTRAQ multiplex tandem mass spectrometry in the detection of the expression of hepatocyte growth factor （HGF） in patients with invasive breast cancer.Methods A total of 35 patients with breast cancer and 30 healthy subjects were selected from January 2014 to October 2016 in this hospital,the expression of serum HGF in breast cancer patients with different clinical stages and healthy subjects was analyzed by iTRAQ labeling,mass spectrometry,library searching and Scqffold software,and the differential expression of HGF was verified by Western blot.Results A total of 237 proteins were identified in the serum samples of this study,and 89 proteins with strict quantitative criteria,17 differentially expressed proteins,included HGF,were screened for breast cancer patients and healthy controls.iTRAQ markers showed that the expression level of serum HGF in different clinical stage of breast cancer patients was significantly higher than that in healthy subjects （P〈0.05）.The results of Western blot showed that the relative expression level of serum HGF in breast cancer patients was significantly higher than that in healthy subjects （P〈0.05）.Conclusion iTRAQ multiplex tandem mass spectrometry is useful for the detection of breast cancer patients with high expression of HGF,which is of great significance in guiding the clinical treatment of breast cancer.