镉胁迫对大麻幼苗生长及生理生化影响

Effect of Growth and Physiology-Chemistry of Hemp (Cannabis sativa) Seedlings under Cadmium Stress

以3个主栽品种大麻(云麻1号、庆大麻、皖大麻1号)为材料,研究了不同镉胁迫浓度(5、20、50μmol/L)对其种子萌发的影响。以"云麻1号"大麻为材料,研究了不同镉胁迫浓度(5、20、50μmol/L)对大麻幼苗生长、根与叶3,3'–二氨基联苯胺(DAB)染色、丙二醛(MDA)含量以及抗氧化酶活性等的影响。结果显示:种子萌发率随镉浓度升高而降低,发芽率依次为:云麻1号>庆大麻>皖大麻1号。"云麻1号"大麻幼根伸长在CdCl_2处理下较对照显著降低,高浓度CdCl_2处理,诱导脂质过氧化,染色着色深,大麻幼根与叶片中MDA含量在20、50μmol/L CdCl_2处理时较对照显著升高。此外,镉胁迫诱导"云麻1号"大麻幼根与叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)活性呈现先升高后降低趋势。结果表明:镉胁迫抑制大麻种子萌发、抑制"云麻1号"大麻幼根伸长;染色着色深、MDA含量增加可能与镉胁迫引起脂质过氧化,破坏细胞膜完整性有关;此外,大麻幼根与叶在镉胁迫下可通过提高抗氧化酶SOD、POD活性来清除活性氧的产生,降低脂质过氧化,保护自身免遭氧化损伤。

Three main cultivars of Hemp （Yunma No.1,Qingdama,Wandama No.1） were employed to analyze changes of seed germination in response to CdCl_2 stress with different concentrations （5 μmol/L,20 μmol/L and 50 μmol/L）.The Hemp of ＂Yunma No.1＂was used to study seedling growth,3,3＇-diaminobenzidine staining on roots and leaves,MDA content,and physiological properties in response to cadmium stress with different concentrations （5 μmol/L,20 μmol/L and 50 μmol/L）.The results shows： The rate of seed germination decreased with the increase of cadmium concentration,and the germination rate was as follows： Yunma No.1 Qingdama Wandama No.1.The root elongation of ＂Yunma No.1＂decreased remarkably under each CdCl_2 concentration in comparison with control;meanwhile,high concentration of CdCl_2 induce lipid peroxidation,deep staining,and the MDA content of root and leaves was significantly increased at 20 μmol/L,50 μmol/L CdCl_2 treatment compared with control.In addition,cadmium stress induced the activity of SOD and POD in roots and leaves of＂Yunma No.1＂which increased first and then decreased.In conclusion,cadmium stress inhibited the seed germination and the seedlings elongation of ＂Yunma No.1 ＂; deep staining and the incresing of MDA content may be associated with lipid peroxidation,destruction of cell membrane integrity caused by cadmium stress.Additionally,the root and leaves of hemp can reduce the activity of reactive oxygen species,decrease the lipid peroxidation and protect itself from oxidative damage by increasing the activities of SOD and POD.