过氧乙酸与异噻唑啉酮合剂对黄丝藻藻华的抑制及南美白对虾养殖安全性研究

Combined inhibition effects of peracetic acid and isothiazolone on alga Tribonema sp. bloom and their safety on Pacific white leg shrimp Litopenaeus vannamei

为寻找一种环保、高效、安全的抑藻产品,并保证用药安全,利用过氧乙酸(PAA)和异噻唑啉酮(Isothiazolone)配伍对黄丝藻Tribonema sp.进行了藻华抑制效果的研究,采用静态生物毒性试验的方法,研究了配伍用药对池塘中南美白对虾仔虾(0.043 5 g±0.000 8 g)和幼虾(2.576 8 g±0.024 0 g)的毒性。结果表明:过氧乙酸和异噻唑啉酮配伍时具有协同作用,可以提高抑藻效果,过氧乙酸和异噻唑啉酮质量浓度比为1.00 mg/L∶0.60 mg/L(配比5∶3)时除藻效果最优,96 h时对黄丝藻的抑制率可达97.40%,协同指数为0.14,对黄丝藻超氧化物歧化酶(SOD)和过氧化氢酶(CAT)具有先诱导后抑制的效果,可使丙二醛(MDA)大量积累;过氧乙酸和异噻唑啉酮配伍对南美白对虾仔虾和幼虾的安全质量浓度比分别为0.21 mg/L∶0.12 mg/L和1.17 mg/L∶0.70 mg/L。研究表明,过氧乙酸与异噻唑啉酮配伍对池塘中的黄丝藻藻华具有良好的抑制效果,但在仔虾养殖阶段要谨慎使用,避免产生药害。

The combined inhibition effects of peracetic acid and isothiazolone on alga Tribonema sp. bloom and acute toxicity of both chemicals to Pacific white leg shrimp Litopenaeus vannamei with body weight of（ 0. 043 5 ±0.000 8） g and（ 2.576 8±0.024 0） g were studied by a static method to search a safe,environmental friend and efficient algae inhibiting products. The results showed that synergistic alga-removal effects of both peracetic acid and isothiazolone were observed,the best at proportion of peracetic acid to isothiazolone = 5 ∶ 3,with the alga Tribonema sp. bloom inhibition rate of 97.40% and the synergistic index of 0.14 in 96 h. The activities of superoxide dismutase（ SOD） and catalase（ CAT） re were shown to be elevated first and then declined,and the content of malondialdehyde（ MDA） to be increased in the alga exposed to the drugs. The safe concentration was found to be the ratio peracetic acid to isothiazolone = 0.21 mg/L ∶ 0.12 mg/L for the Pacific white leg shrimp post larvae and 1.17 mg/L ∶0.70 mg/L for the juveniles. The findings indicate that peracetic acid and isothiazolone are applied in Pacific white leg shrimp culture ponds with causation even though they have good inhibitory effect on Tribonema bloom.