SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus

为探索禽网状内皮组织增生病病毒(REV)感染对雏鸡中枢免疫器官细胞凋亡及其相关酶活性和基因表达的影响,将70只1日龄SPF雏鸡随机分为REV感染组(I)和对照组(C),应用酶活性检测、荧光定量PCR、ELISA、TUNEL等方法对中枢免疫器官细胞的Caspase-3和Caspase-9活性、Bcl-2mRNA及其蛋白质表达和凋亡细胞的动态变化进行了检测。结果发现,SPF雏鸡感染REV后,其胸腺中Caspase-3和Caspase-9活性、Bcl-2mRNA及其蛋白质含量、凋亡细胞数量均于7~28d内不同程度(P<0.05或P<0.01)高于对照组雏鸡;法氏囊中上述被检指标均于7~35d内显著(P<0.05)或极显著(P<0.01)高于对照组雏鸡。研究表明REV感染引起SPF雏鸡中枢免疫器官Caspase-3和Caspase-9活性增强、Bcl-2mRNA及其蛋白质表达上调所致的细胞凋亡与雏鸡免疫器官功能抑制密切相关。

The research was conducted to study the effects of reticuloendotheliosis virus （REV） on cell apoptosis, enzyme activities and expression of relevant factors in immune organs of chicks. Seventy one-day old SPF chicks were randomly selected into REV infection groups （I） and control groups （C）. The methods of enzyme activities detection kit, RT-PCR, ELISA and TUNEL were applied to detect activities of Caspase-3 and Caspase-9, expression of Bcl-2 and cell apoptosis in central immune organs. The results showed that the detected indexes in thymus of SPF chicks infected with REV were significantly higher than control groups at 7 to 28 days after infection. The indexes in bursa of Fabricius of SPF chicks infected with REV were significantly increased than control groups at 7 to 35 days after infection. These data indicate that cell apoptosis caused by enhancement of Caspase-3 and Caspase-9 activities, up-regulated expression of the Bcl-2 mRNA and protein in central immune organs of SPF chicks infected with REV, are closely related to immune function suppression.