鸡脾转移因子对鸡肠道抗氧化能力的影响

The Influence of Chicken Spleen Transfer Factor on Antioxidant Capacity of Chicken Intestine

旨在通过分析鸡脾转移因子(TF)对鸡肠道抗氧化能力的影响,探讨其调控鸡生长、免疫的作用机制。选用1日龄SPF鸡100羽,自5日龄开始在饮水中加入鸡脾转移因子,饲喂1周,停喂1周,饲喂分为4个剂量组,分别为0.05(最低剂量组)、0.10(低剂量组)、0.25(中剂量组)、1mL·只-1(高剂量组)剂量组及正常对照组,饲养至12日龄(连续用药1周)及19日龄(停喂1周)时各取50羽,分别称量其体重,并测量十二指肠、空肠、回肠和直肠的抗氧化能力。结果表明,口服鸡脾转移因子1周,就显示出升高体重的趋势,但是效果尚不明显。高剂量组可显著增加12日龄雏鸡十二指肠、空肠、回肠、直肠SOD(17.07%~31.54%)、CAT(16.98%~25.77%)、GSH-PX(13.99%~23.59%)的活力,以及总抗氧化能力T-AOC(16.48%~43.16%),降低各段MDA含量(P<0.05);中剂量组显著增加各肠段的T-AOC和SOD活性、CAT活力(回肠除外)、GSH-PX活力(十二指肠除外);且低、中剂量组均显著降低各肠段的MDA含量(P<0.05)。停喂一周后,尽管各剂量组对各肠段T-AOC、CAT、GSH-PX、SOD、MDA活性的改变较12日龄有不同程度的降低,但是某些肠段较对照组仍然作用显著(P<0.05)。结果提示,鸡脾转移因子作为新型饲料添加剂能显著提高鸡肠道抗氧化应激能力。

The experiment was conducted to study the effect of chicken spleen transfer factor （TF） on intestine antioxidant capacity and the mechanism on production and immunity ability of chicken. One hundred chickens of 1-day-old were selected and were divided into 5 groups. Four TF groups including 20 chickens each received TF treatment as follows： 0.05 mL （Minimum dose group）, 0.10 mL （Low dose group）, 0.25 mL （Medium dose group）, 1 mL （High dose group） per chicken TF were given for one week by drinking water every day from 5-days-old to 12 daysold, respectively, and then fed without TF to 19-days-old. Group 5th was normal control group. Fifty chickens were killed at 12-days-old and 19-days-old, respectively. The chickens were weigh-ted, and the antioxidant capacity of the duodenum, jejunum, ileum and rectum were measured. The results showed that one week＇ treatment of TF showed an increased tendency of body weight, yet the effect was not obvious. However, the high TF dose group significantly increased the activity of SOD （17.07%-31.54%）, CAT （16.98%-25.77%）, GSH-PX （13.99%-23.59%） and T-AOC （16.48%-43.16%） of duodenum, jejunum, ileum and rectum, but significantly reduced the activity of MDA （P〈0.05） in intestine. The activities of T-AOC, SOD in the intestinal segments, CAT （except for ileum） and GSH-PX （except for duodenum） were significantly increased in the middle dose groups （P〈0.05）, but the content of MDA was decreased significantly in the low and middle dose groups （P〈0.05）. At 19 days old, the changes of T-AOC, CAT, GSH-PX, SOD and MDA in each intestinal group were decreased compared with 12 days old, but there was still significant in individual intestinal segment （P〈0.05）. The results indicate that the TF, as a new feed additive, has a significant role in improving anti-oxidative stress ability.