结直肠癌中hsa_circ_0006156 RNA的低表达及其临床意义

Decreased expression of hsa_circ_0006156 in colorectal cancer and its significance

目的探讨hsa_circ_0006156基因在结直肠癌(colorectal cancer,CRC)中的表达水平与临床意义。方法利用荧光定量PCR技术检测2014年12月-2015年12月于本院行手术治疗的31例结直肠癌病人的肿瘤组织和癌旁组织中hsa_circ_0006156的表达水平,并结合临床病理资料分析其临床意义。利用Blast、Bedtool等软件预测分析hsa_circ_0006156与micro RNA的相互作用。结果 hsa_circ_0006156在结直肠癌组织中表达水平显著低于癌旁正常组织(P=0.004)。相较于癌旁正常组织,hsa_circ_0006156在24例病人癌组织中(77.4%)表达下调,7例(22.6%)表达上调,hsa_circ_0006156表达水平与肿瘤大小相关(P=0.042)。hsa_circ_0006156表达诊断癌组织与癌旁组织的受试者工作特征曲线(receiver operating characteristic curve,ROC)的曲线下面积(area under curve,AUC)为72.3%(95%CI:0.592~0.853),灵敏度和特异度分别为71%和77.4%。hsa_circ_0006156序列上含有分别针对hsa-mi R-5011(n=24)、hsa-mi R-4775(n=22)、hsa-mi R-4445(n=20)、hsa-mi R-627(n=12)等mi RNA的结合位点(binding sites)。结论 hsa_circ_0006156在结直肠癌组织中表达水平下调,可作为结直肠癌的诊断分子标记物。其可能作为"mi RNA海绵"(mi RNA sponge)发挥转录后调控作用。

Objective To investigate the expression of hsa_circ_0006156 in colorectal cancer（CRC） and its significance. Methods The expression of hsa_circ_0006156 in CRC tissues and adjacent normal tissues in 31 patients who underwent surgical treatment in our hospital from December 2014 to December 2015 was detected by real-time fluorescence-based quantitative PCR. The clinicopathological data were collected and analyzed to study its clinical significance. The interaction between hsa_circ_0006156 and micro RNAs was predicted and analyzed by bioinformatic software such as Blast and Bedtools. Results Compared with the normal tissues, hsa_circ_0006156 was significantly down-regulated in cancer tissues with low expression in 24 patients（77.4%）, while it was up-regulated in 7 patients（22.6%）, and its expression was correlated with tumor size（P=0.042）. ROC curve was constructed for differentiating CRC tissues from normal tissues. The AUC（area under curve） was up to 72.3%（95% CI： 0.592-0.853）, and the sensitivity and specificity of hsa_circ_0006156 were 71% and 77.4%, respectively. hsa_circ_0006156 had binding sites of hsami R-5011（n=24）, hsa-mi R-4775（n=22）, hsa-mi R-4445（n=20）, and hsa-mi R-627（n=12）. Conclusion The expression of hsa_circ_0006156 is down-regulated in CRC and is a potential diagnostic biomarker for clinical diagnosis. hsa_circ_0006156 may act as mi RNA sponge in post-transcriptional regulation.