红光发射分子转子的设计与合成

Design and Synthesis of Red Emissive Molecular Rotor

粘度对细胞微环境的维持非常重要。检测细胞内的粘度一般采用分子转子,而目前大多数的分子转子发射波长较短,不利于生物成像,为此我们设计了基于扭转分子内电荷转移(TICT)机理,通过共轭双键连接吸电子基和给电子基的红光发射的分子转子2-(2-(4-氨基苯乙烯基)-4-H-吡喃-4-亚基)-丙二腈(DCM-NH_2)。DCM-NH_2的最大发射波长为631 nm,属于远红光,能有效减少生物背景,提高成像信噪比。该探针对粘度有很好的响应,具有非常宽的线性响应范围(0.6~458.6 c P),同时也具有较高的灵敏度。

Viscosity is very important to maintain cell microenvironment. Recenthy, fluoresent molecular rotors has been developed to determine the viscosity in solutions or biological fluid. However, most of molecular rotors emit at short wavelength, results in shallow imaging. To solve this problem, and based on twisted intramolecular charge transfer（TICT） mechanism, we designed a red emissive molecular rotor （E）-2-（ 2- （ 4-aminostyryl ）-4 H-chromen-4-ylidene ） malononitrile （ DCM-N H2 ） by con] ugating electron accepter and electron donor through a C- C double bond. DCM-NH2 emits at red region, which can decrease background fluorescence and gain a higher signal-to-noise（S/N） ratio. The molecular rotor can respond to viscosity selectively and sensitively in a broad viscosity range （0.6-458.6 cP）.