能量调控下草莓果实衰老过程中活性氧与超微弱发光的关系

Relationship Between Ultraweak Photon Emissio and Reactive Oxygen Under Regulating Energy in Ageing Process of Strawberry Fruit

以‘红颜’草莓果实为试材,采用呼吸链解偶联剂2,4-二硝基苯酚(DNP)和三磷酸腺苷(ATP)处理草莓果实,以蒸馏水浸泡为对照,对比分析抑制能量生成和增加能量条件下草莓果实衰老过程中超微弱发光(ultraweak photon emissio,UPE)和活性氧的变化及二者之间的关系。结果表明:在草莓果实采后衰老过程中,DNP处理、ATP处理和对照的超氧阴离子(O_2~·)产生速率、丙二醛(MDA)含量和相对电导率均持续上升;同时三者的UPE强度均整体下降。在整个果实衰老过程中,DNP处理的O_2~·产生速率、MDA含量和相对电导率均高于对照,ATP处理后三者均低于对照。相关性分析也表明,UPE强度与活性氧水平呈显著负相关。由以上结果可见,抑制ATP生成情况下加剧了草莓果实活性氧上升积累,同时加剧了UPE强度下降;增加ATP则减缓了活性氧上升积累,同时减缓了UPE强度下降。UPE强度随着活性氧的增加积累而下降。以上结果表示活性氧并不是引发UPE的直接来源,而是间接影响了UPE。

The study aimed to understand the change of ultraweak photon emission（UPE） and reactive oxygen, and the relationship between UPE and reactive oxygen by soaking fruit with 2,4-dinitrophenol （DNP） and adenosine triphosphate（ATP） in ageing process of‘Hongyan＇ strawberry fruit. The results showed that, during fruit ageing process, superoxide anion radical （O7）production rates, malondialdehyde （MDA） content and relative electric conductivity increased continuously under three treatments of ATP, DNP and CK. Meanwhile UPE intensity of three treatments decreased. Furthermore, in whole ageing process, O7 production rate, MDA content and relative electric conductivity of DNP treatment were all higher than that of CK but UPE intensity was lower than that of CK. While O^- production rate, MDA content and relative electric conductivity of ATP treatment were all lower than that of CK but UPE intensity was higher than that of CK. Correlation analysis showed that UPE intensity had a significantly negative correlation with reactive oxygen level. Above results stated that inhibiting ATP intensified the accumulation of reactive oxygen and meanwhile intensified the decline of UPE intensity. However, increasing ATP relieved the accumulation of reactive oxygen and the decline of UPE intensity. UPE intensity declined along with the accumulation of reactive oxygen. The results indicated that reactive oxygen was not the direct source for UPE but could effect UPE indlrectly.