刺五加转录因子基因的挖掘

Exploring Transcription Factors in Eleutherococcus senticosus

以刺五加嫩叶为试材,采用二代测序方法Illumina HiSeq 4000进行转录组测序和生物信息学方法,找出其转录因子,并随机挑选4个不同家族的转录因子,针对6个生长时期和3个器官的样本,使用实时荧光定量PCR确认转录因子的正确性。结果表明:刺五加转录组共计8.34Gb,拼接得到77 087条Unigenes,有485个转录因子,分类于36个家族。通过qRT-PCR分析,Unigene 49771、Unigene 22314、Unigene 13139和Unigene18837在不同生长发育时期和器官中表达差异极显著(P<0.01)。

The leaves of Eleutherococcus senticosus were used as experimental materials, the high- throughput sequencing technology （Illumina HiSeq 4000） was used to sequence the transcriptome,in order to find transcription factors from the transcriptome with the hioinformatic way. And then the qRT-PCR was used to analysis correctness of transcription factors with 6 kinds of stages and 3 kinds of tissues of E. senticosus. The results showed that the transcriptome of E. senticosus was 8. 34 Gb, and it contained 77 087 Unigenes. There were 485 transcription factors in it,which involved in 36 families. By qRT-PCR analysis, the difference of Unigene 49771, Unigene 22314, Unigene 13139 and Unigene 18837 was highly significant in different growth stages and organs （P〈0.01）.