脂多糖对星形胶质细胞Tet1和Tet2表达的影响

Effects of LPS on the expression of Tet1 and Tet 2 in astrocytes

目的探讨脂多糖（LPS）对人脑星形胶质母细胞瘤细胞（U87）10—11易位酶1（Tetl）、10—11易位酶2（Tet2）表达的影响。方法用LPS和（或）氯胺酮刺激星形胶质细胞，Trizol法提取总RNA，检测Tetl、Tet2mR．NA表达的变化。将细胞分为5组：对照组，不同浓度LPS组（1mg／L、10mg／L，分别标记为L1组、L10组），氯胺酮组（K组），LPS加氯胺酮组（L1＋K组）。结果与对照组比较，LPS组TetlmRNA的表达显著减少，L1组尤为显著，Tet2mRNA的表达无明显变化；而L1＋K组与L1组比较TetlmRNA的表达明显增加。结论LPS可以诱导人脑星形胶质母细胞瘤细胞TetlmRNA表达的下调，氯胺酮可以逆转这一现象。

Objective To investigate the effects of lipopolysaccharide （LPS） on the expression of ten - eleven translocation enzyme 1 （ Tetl ） and ten - eleven translocation enzyme 2 （Tet2） in astrocytes, Methods Astroeytes were stimulated with LPS and/or ketamine. Their total RNA was extracted by Trizol method to detect the levels of Tet1 and Tet2 mRNA. The cells were divided into five groups ： a control group, 1 mg/L and 10 mg/L LPS groups （ Groups L1 and L10） , a ketamine group （Group K） , and a LPS plus ketamine group （Group L1 ＋ K）. Results Compared with the control group, the level of Tetl mRNA in cells exposed to LPS was significantly decreased, especially Group L1. No sig- nificant difference was found in Tet2 mRNA expression. In contrast, the amount of Tetl mRNA was significantly increased in Group L1 ＋ K and Group L1. Conclusion LPS can induce the down - regulation of Tetl mRNA expression in human astroeytoma cells which can be reversed by ketamine.