抗苗勒管激素及其Ⅱ型受体在子宫颈鳞状细胞癌中的表达

Expression of anti - mullerian hormone and its type Ⅱ receptor in cervical squamous cell carcinoma

目的观察抗苗勒管激素（anti—mullerian hormone，AMH）及其Ⅱ型受体（anti—mullerian hormonere．ceptorⅡ，AMHR-Ⅱ）在子宫颈鳞状细胞癌（cervical squamous cell carcinoma，CSCC）组织中的表达及分布，探讨二者在CSCC发生发展中的作用。方法应用免疫组织化学方法及实时荧光定量PCR法检测30例CSCC、30例子宫颈高度鳞状上皮内病变（high—grade squamous intraepithelial lesions，HSIL）、30例子宫颈低度鳞状上皮内病变（low—grade squamous intraepithelial lesions，LSIL）及30例慢性子宫颈炎组织（chronic cervicitis，CC）样本中AMH及AMHR-Ⅱ的表达。结果与CC组比较，CSCC组和HSIL组中AMH和AMHR-Ⅱ阳性细胞数和mRNA表达水平降低，差异均有统计学意义（P〈0．01），LSIL组无显著性差异（P〉0．05）。与LSIL组比较，CSCC组和HSIL组中AMH和AMHR—Ⅱ阳性细胞数和mRNA表达水平降低，差异均有统计学意义（P〈0．01）。CSCC组与HSIL组比较，差异无统计学意义（P〉0．05）。结论AMH及AMHR—Ⅱ与子宫颈鳞状细胞癌的发生、发展有一定关系，而且随着子宫颈病变进展呈递减趋势，有望成为子宫颈癌早期诊断、早期治疗的生物学指标。

Objective To observe the expression and distribution of anti - mullerianhormone （AMH） and its type Ⅱ receptor （AMHR - Ⅱ ） in cervical squamous cell carcinoma （CSCC）, and discusses the role of AMH and AMHR - Ⅱ in the development of cervical sqnamous cell carcinoma. Methods The expression of AMH and AMHR -Ⅱ were examined by immunohistochemistry and real - time quantitative PCR using 30 CSCC samples, 30 samples of high - grade squamous intraepithelial lesions （ HSIL）, 30 samples of cervical low - grade squamous intraepithelial lesions （LSIL） and 30 samples of chronic cervicitis （CC） tissues. Results Compared with Group CC, Groups CSCC and HSIL showed remarkably decreases in the positive cell number and mRNA level of AMH and AMHR - Ⅱ（ P 〈 0.01 ）. Compared with Group LSIL, Groups CSCC and HSIL showed remarkably decreases in the positive cell number and mRNA level of AMH and AMHR- Ⅱ （P 〈0.01 ）. No statistical difference was found as to the positive cell number and mRNA level of AMH and AMHR - Ⅱ between Groups CC and LSIL, and Groups CSCC and HSIL （ P 〉 0.05 ）. Conclusions The expression of AMH and AMHR -Ⅱ is closely related to the development of cervical squamous cell carcinoma, which are decreased as cervical lesions develop. AMH and AMHR - Ⅱ are expected to be biological indicators for early diagnosis and treatment of cervical squamous cell carcinoma.