不同激素配比对南非叶叶片愈伤组织诱导的影响

Effects of Different Hormone Combinations on Callus Induction of Leaf Explant from Vernonia amygdelina

南非叶是热带地区一种重要的中草药材,主要以扦插繁殖为主,为拓宽繁殖途径,实验以南非叶外植体叶为材料,以不同激素浓度配比诱导其愈伤组织,筛选最佳诱导浓度组合。结果表明:(1)NAA和6-BA各5个浓度梯度组合中,1.5 mg/L NAA+1.5 mg/L 6-BA+0.5 g/L活性碳愈伤组织诱导率最高,为88.3%。即当6-BA为定量时,随着NAA浓度的增加,诱导率也逐渐提高;当NAA浓度为1.5 mg/L时,诱导率达到最佳效果,如继续增加NAA的浓度反而会下降。如单独使用6-BA时,则外植体不分化。(2)在NAA和2,4-D组合中,1.5 mg/L NAA+2.0 mg/L 2,4-D+0.5 g/L活性碳愈伤组织诱导率最高,达95%。当2,4-D浓度大于0.5 mg/L,单独使用时,外植体也可诱导分化,增殖效果随着2,4-D浓度的增加而增加。

Bitter leaf （Vernomia amygdelina） is an important Chinese medicinal plant in the tropical areas, and is propagated mainly by cutting. In order to find more ways for propagation, the leaf explant of the bitter leaf was induced into calli with different hormones at different concentrations to screen optimum hormone combinations. The results showed that the callus inducing rate was 88.3%, the highest in the 5 hormone combinations of NAA and 6-BA when the explant was treated with the hormone combination of 1.5 mg/L NAA ＋ 1.5 mg/L 6-BA ＋ 0.5 g/L. When the 6-BA concentration was not changed, the callus inducing rate increased with the concentration of NAA. The callus inducing rate was the highest when the concentration of NAA was 1.5 mg/L, but decreased when the NAA concentration increased. The leaf explant did not differentiate when only treated with 6-BA. In the hormone combinations of NAA and 2,4-D the combination of 1.5 mg/L NAA ＋ 2.0 mg/L 2,4-D ＋ 0.5 g/L gave the highest callus inducing rate （95%）. When the 2,4-D concentration was greater than 0.5 mg/L, the 2,4-D alone could induce the explant into differentiation, and the proliferation effect increased with the 2,4-D concentration.