牛蒡苷元对鱼藤酮致大鼠脏器损伤的保护作用

Protective effect of arctigenin on organ damage of rotenone-induced parkinson rats

目的观察鱼藤酮对帕金森模型大鼠各脏器的损伤,探讨牛蒡苷元对其缓解与治疗作用。方法采用颈背部皮下注射鱼藤酮葵花油乳化液4周的方法建立大鼠帕金森模型,并灌胃给予不同剂量的牛蒡苷元进行干预治疗,观察各组大鼠脏器指数。采用HE染色法和电镜法观察大鼠脏器损伤程度,检测血清中丙氨酸转移酶(alanine aminotransferase,ALT)、肌酐(creatinine,CRE)与尿素氮(urea nitrogen,BUN)的含量。结果模型组大鼠的肝指数和肾指数与正常组相比显著升高,血清中ALT与BUN的含量显著升高(P≤0.01),肝脏切片显示肝脏内存在着中央静脉扩张、慢性炎症细胞浸润等病理改变,肾皮质和髓质内均有慢性出血和炎性浸润,并可见肾小球固缩。牛蒡苷元治疗后肝肾指数显著降低,血清中ALT与BUN明显降低(P≤0.01),肝肾相应损伤有所缓解。结论牛蒡苷元能有效抑制鱼藤酮所致的大鼠肝脏和肾脏肿大,可缓解和治疗鱼藤酮所引起肝和肾的损伤。

Objective To explore the organ damage caused by rotenone in Parkinson rats and protective effect of arctigenin on organ damage. Methods After establishing dopaminergic neurons in substantia nigra injury model by injecting rotenone subcutaneously on back of neck and back and treating with different doses of arctigenin,hematoxylin-eosin （HE）staining was used to observe the morphological changes in liver and kidney. Weigh the liver and kidey and calculate the liver index and kidney index. Serum alanine transaminase （ ALT）, creatinine（CRE） and blood urea nitrogen（BUN） were measured to assess kidney and liver function. Results Rotenone can induce the content of BUN and ALT in blood increasing, kidney and liver index increasing and the histopathologic examination showed different degrees of lobes of liver and renal tubular injury. After treating, arctigenin significantly prevent the increase in liver index, kedney index, serum ALT and BUN caused by rotenone in mice. Histopathological examinations further confirmed these results. ConclusionsThe results of this study showed that the arctigenin could, to some extent, protect mice from rotenone-induced liver and kidney injury.