普通大蓟马不同地理种群的分子鉴定及酶活测定

Molecular Identification and Enzyme Activity Determination of Different Geographical Populations of Megalurothrips usitatus(Bagnall)

普通大蓟马Megalurothrips usitatus(Bagnall)是影响海南豇豆品质的重要害虫之一。笔者在采集不同地理种群的基础上,用试剂盒法提取其总DNA,对mt DNA COⅠ基因序列进行测序分析,并构建系统发育树;用分光光度计法进行酶活比较分析。结果表明,普通大蓟马不同地理种群之间的遗传距离非常小,并没有随地理位置的变化产生较大的遗传分化;抗氧化酶系活力测定中,不同地理种群的普通大蓟马GST活力差异较大,SOD活力不存在显著性差异,个别地理种群CAT活力与其他地理种群存在显著性差异。

Bean flower thrips, Megalurothrips usitatus （ Bagnall）, is one of the major pests in cowpeas grown in Hainan, China, affecting the quality of cowpeas. From different geographical populations of M. usitatus collected, the total DNA of M. usitatus was extracted by using TIANamp Genmmic DNA Kit, and the mtDNA COI gene sequence was sequenced, based on which a phylogenetic tree was constructed. The enzyme activity was de- termined by using spectrophotometer. The results showed that the genetic distance between the different geo- graphical populations was very small, and that there was no significant genetic differentiation with the change of geographical location. Determination of antioxidant enzyme activity showed significant difference in glutathione-S- Transferase （GST） activity between different geographic populations. There was no significant difference in SOD activity between different geographic populations. There was significant difference in CAT activity between indi- vidual populations and other geographic populations.