升血颗粒含药血清对人白血病K562/ADR细胞多药耐药的逆转作用

Reverse Action of Serum Containing ShengXue Granules on Multidrug Resistance of Human Leukemia K562/ADR Cells

目的:观察升血颗粒(PG)含药血清对人白血病K562/ADR细胞多药耐药性的逆转作用,并探讨其逆转机制。方法:采用MTT法测定细胞的药敏性及耐药逆转性,应用流式细胞仪检测非细胞毒性的含药血清处理后K562/ADR细胞内阿霉素(ADM)的浓度。结果:低、中、高剂量含药血清对K562/ADR细胞无明显细胞毒性。低、中、高剂量含药血清作用后,ADM对K562/ADR细胞的半数抑制浓度(I C50)显著下降,逆转倍数分别为1.34、1.71、1.82倍;ADM外渗试验显示,低、中、高剂量含药血清处理后K562/ADR细胞内ADM浓度显著增加,增加倍数分别为1.41、1.67、2.04倍。结论:升血颗粒含药血清对人白血病K562/ADR细胞耐药性有一定的逆转作用。

Objective： To observe the reverse action of serum containing ShengXue granules on multidrug resistance（MDR） of human leukemia K562/ADR cells, and discuss its reversal mechanism. Methods： Drug sensitivity and drug resistance reverse of the cells were detected by using MTT method, and the concentrations of amycin in K562/ADR cells in non cytotoxicity serum containing the drugs after treating were detected by using flow cytometry.Results： High, moderate and low dosage serum containing the drugs showed no obvious cytotoxicity to K562/ADR cells, after dealing with high, moderate and low dosage serum containing the drugs, IC50 of ADM to K562/ADR cells decreased significantly, reversal indexes were 1.34, 1.71 amd 1.82 times; ADM extravasation test demonstrated that ADM concentrations increased obviously in K562/ADR cells after dealing with low, moderate and high dosage serum containing the drugs, the increasing times were 1.41, 1.67 and 2.04 times. Conclusion： The serum containning ShengXue granules shows certain reverse action to human leukemia K562/ADR cells.