miR-30a-3p对食管鳞癌ECA-109细胞侵袭和增殖能力的影响及生物信息学分析

Roles of miR-30a-3p in invasion and proliferation of ESCC cells ECA-109 and bioinformatics analysis

为探讨miR-30a-3p对食管鳞癌细胞生物学行为的影响及机制,miR-30a-3p转染ECA-109细胞后,实时荧光定量PCR检测各组细胞miR-30a-3p表达水平,Transwell小室检测细胞侵袭能力,CCK-8检测细胞增殖能力,通过生物信息学预测miR-30a-3p的靶基因,分析靶基因集合的基因功能及通路富集。结果显示,miR-30a-3p抑制ECA-109细胞侵袭能力(P<0.05),但对增殖能力的影响无统计学意义(P>0.05)。利用系统的生物信息学预测得到与ESCC相关的靶基因77个,其中在食管癌中上调的差异基因42个,下调的差异基因35个。miR-30a-3p的靶基因多集中于迁移、黏附连接、外泌体及蛋白代谢等过程;靶基因显著富集于Ras信号通路。由此可知:miR-30a-3p可抑制ESCC细胞的侵袭能力,分析得出的参与侵袭迁移及其他功能的靶基因为进一步研究提供了方向。

To investigate the invasion and proliferation effects of miR-30a-3p in ESCC, to get target genes of miR-30a-3p inESCC by bioinformatics approaches, and to analysis the molecular functions in effects. Transwell invasion assay and CCK-8proliferation assay were used to detect the invasion and the proliferation after transfecting ECA-109 with miR-30a-3p. Targetgenes of miR-30a-3p were predicted by bioinformatics, combined with relevant literature, ESCC GEO database and predictionsoftware. DAVID database was used to analyze the GO （Gene Ontology） annotation and KEGG （Kyoto encyclopedia of genes andgenomes） signal pathway enrichment respectively. The results showed that overexpression of miR-30a-3p could inhibit theinvasion（ 〈0.05）, but had no effect on the proliferation（ 〉0.05）. There were 77 target genes in ESCC got by bioinformaticsapproaches, with 42 up-regulated genes and 35 down-regulated genes. GO of these genes were significantly involved inmigration, adherent junction, exosome and protein metabolism. These target genes were mainly enriched in Ras signalingpathway. It is concluded that Mir-30a-3p can inhibit the invasion of ESCC, its target genes and their ontology and pathwayprovide directions for further research.