微生物修复重金属污染土壤探究

Research on microbial remediation of heavy metal contaminated soil

利用微生物对重金属的钝化作用,制备微生物钝化剂并应用于重金属污染土壤的治理。通过研究重金属污染土壤中微生物的性质,筛选出能有效处理土壤中重金属污染的优良菌种CQ7与FQ2;对筛选出的菌株进行表征,并测定其生长曲线,鉴定FQ2为革兰氏阴性杆菌。进一步对筛选出的菌种进行理化条件测定,测定得CQ7与FQ2的最适生长条件均为p H=5、转速180 r/min、温度25℃。经金属耐受和吸附实验,选定FQ2为工程菌,测序确定其为沙雷氏菌属(Serratia marcuscens)。将筛选所得菌种与相应理化条件相结合,制备微生物钝化剂,设计自动化重金属污染土壤修复设备,对其应用前景进行了初步规划。

Inactivation of heavy metals by microorganisms is used for preparing the microbial passivating agent and applying it to the treatment of heavy metal contaminated soil. Through the study on the properties of microorganisms in heavy metal contaminated soil,the fine strains CQ7 and FQ2 which can effectively deal with heavy metal pollution in soil are screened out. The screened strains are characterized,their growth curves are measured,and FQ2 is identified as gram negative bacillus. The physical and chemical conditions of the screened strains are further determined. The optimum conditions for the growth of CQ7 and FQ2 are p H = 5,the rotational speed is 180 r/min and the temperature is 25℃. Through the metal tolerance and adsorption experiments,FQ2 is selected as the engineering bacteria and identified as the Serratia marcuscens by the sequence test. In combination of the screened strains with the corresponding physical and chemical conditions,the microbial passivator is prepared,and the automatic remedial equipment for the heavy metal contaminated soil is designed whose application prospect is primarily planned.