模拟冻融对高山土壤微生物数量和活性的影响

Effects of simulation freeze-thaw cycle on the number of the alpine soil microbial and microbial activity

目的:全面了解冬季高山土壤循环过程,为全球气候变化下生物种群和分布的变化提供技术支持.方法:采用室内模拟冻融的方法,研究了不同冻融处理(-2℃,-2~2℃,-20~2℃)对土壤微生物数量和活性的影响.结果:研究发现,持续的冻结(-2℃)降低了土壤细菌数量、微生物总数量和微生物生物量碳氮含量.剧烈的冻融(-20~2℃)降低了蔗糖酶活性,而温和的冻融(-2~2℃)则增加了细菌数量、微生物总数量和纤维素酶、过氧化氢酶活性.结论:适当的冻融可以通过改善土壤微生物数量和群落结构,从而对土壤营养物质的转化和植物群落的构建产生实质性的影响.

Aim： In order to understand the alpine soil ecology process,to provide technical support for the change of biological population and distribution under the global climate change. Methods： The effects of different freezing-thawing treatments（-2 ℃,-2 ~ 2 ℃,-20 ~ 2 ℃） on the number of soil microorganism and activity were studied by simulated freeze-thaw in lab. Results： The results showed that the continuous frozen state reduced the number of bacteria, the number of total microbe, and microbial biomass carbon and nitrogen contents. The severe freeze-thaw decreased the activity of invertase, while moderate freeze-thaw increased the number of bacteria, the total number of microorganisms and the activity of cellulose and catalase. Conclusion： The results suggest that the appropriate freeze-thaw can improve the number of soil microbial and community structure,and thus have a substantial impact on the transformation of soil nutrients and the construction of plant communities.