基于活性追踪法的商陆细胞毒活性物质研究

Study on the Cytotoxic Active Substance of Phytolacca Americana L. Based on Activity Tracing Method

[目的]初步探明中药商陆卤虫毒活性部位。[方法]干燥的商陆粉末以70%乙醇、80℃提取3 h,减压回收乙醇获商陆乙醇总提物。总提物溶于水,依次以石油醚、乙酸乙酯、正丁醇萃取,回收萃取液获商陆乙醇总提物石油醚相、乙酸乙酯相、正丁醇相及水相,以卤虫毒活性为评价依据,分别配制0.5、1.0、1.5、2.0 mg/m L溶液,测定其卤虫毒活性。[结果]石油醚相剂量组培养6 h后,各浓度组卤虫校正死亡率均达100%。采用正向硅胶柱50∶1石油醚—乙酸乙酯、10∶1石油醚—乙酸乙酯、3∶1石油醚—乙酸乙酯、乙酸乙酯、甲醇梯度洗脱,将石油醚相分成5个组分不同的部位,各部位的活性均低于石油醚相。[结论]商陆卤虫毒活性成分主要集中在石油醚相。

[Objective] The research aimed to proven preliminarily the active parts of Artemia poison of Phytolacca Americana. [Method]The dried powder of Phytolacca Americana was extracted with 70% ethanol and 80 ℃ for 3 h,and the total ethanol extract was obtained by recovering ethanol under reduced pressure. The total extract was dissolved in water,followed by extraction with petroleum ether,ethyl acetate and n-butanol,the ethanol ether phase,ethyl acetate phase,n-butanol phase and water phase of the ethanol total extract of Phytolacca Americana was obtained by recovering the extract. Based on the evaluation basis of the artemia poison activity,the solution of 0. 5,1. 0,1. 5,2. 0 mg/m L were prepared,respectively and their activities were determined.[Result]Petroleum ether phase dose group was cultured after 6 hours,the corrected mortality rate was 100% in each concentration group. The gradient elution was carried out using positive silica column 50∶ 1 petroleum ether-ethyl acetate,10∶ 1 petroleum ether-ethyl acetate,3∶ 1 petroleum ether-ethyl acetate,ethyl acetate,the petroleum ether phase was divided into different parts of the five components,the activity of each part was lower than that of petroleum ether phase.[Conclusion]The active ingredients of Artemia poison of Phytolacca Americana were mainly concentrated in petroleum ether phase.