甘肃产藏药绿绒蒿中总黄酮和总生物碱的含量测定

Determination of Total Flavonoids and Total Alkaloids Content from Tibetan Medicine Meconopsis Produced in Gansu

[目的]测定甘肃产藏药绿绒蒿中总黄酮、总生物碱含量,为其质量评价提供依据。[方法]采用紫外分光光度法,以芦丁为对照,测定总黄酮含量;以罂粟碱为对照,测定总生物碱含量。[结果]绿绒蒿中总黄酮含量测定条件为:60%乙醇回流提取制备供试品溶液,以芦丁为对照,以5%亚硝酸钠、10%硝酸铝显色,在500 nm处测定吸光度;绿绒蒿中总生物碱含量测定条件为:采用氨水碱化处理,95%乙醇超声提取,0.01 mol/L盐酸溶解定容制备供试品溶液,以罂粟碱为对照,溴甲酚绿显色,在416 nm处测定吸光度。甘肃甘南产五脉绿绒蒿中总黄酮含量介于四川产五脉绿绒蒿和青海产五脉绿绒蒿中总黄酮含量之间,总生物碱含量均低于青海产五脉绿绒蒿和四川产五脉绿绒蒿中总生物碱含量。[结论]所建立的绿绒蒿中总黄酮、总生物碱含量测定方法准确、可行、重现性好,可作为绿绒蒿药材质量评价方法的科学依据,试验结果也为甘肃产绿绒蒿药材内在品质提供科学依据。

[Objective] The research aimed to determine the content of total flavonoids and total alkaloids in Meconopsis,and provide the basis for its quality evaluation. [Method]Using ultraviolet spectrophotometry,the contents of total flavonoids were determined by using rutin as control,the content of total alkaloid content was determined by using papaverine as the control. [Result]The content of total flavonoids in Meconopsis was determined as follows： 60% ethanol was refluxed to prepare the test solution,using rutin as the control,with 5% sodium nitrite,10%aluminum nitrate color,the absorbance was measured at 500 nm. The content of total alkaloids in Meconopsis was determined as follows： using ammonia water alkalization treatment,95% ethanol ultrasonic extraction,0. 01 mol/L hydrochloric acid dissolved constant volume preparation of the test solution,using papaverine as the control,bromocresol green color,the absorbance was measured at 416 nm. The content of total flavonoids in Meconopsis quintuplinervia in Gannan of Gansu Province was between that of Sichuan and Qinghai,and the content of total alkaloid was lower than that of Qinghai and Sichuan. [Conclusion]The method is simple,feasible and reproducible,and it can be used as the scientific basis for the quality evaluation of the quality of Meconopsis. The experimental results also provide the scientific basis for the intrinsic quality of the medicinal plants Meconopsis produced in Gansu.