加拿大蓝靛果忍冬组培快繁技术研究

A Preliminary Study of Tissue Culture Technique of Canada's Lonicera edulis

[目的]研究加拿大蓝靛果忍冬组培快繁技术。[方法]以黑龙江省森林植物园选育的加拿大蓝靛果忍冬优良品种J-12的叶芽、花芽、茎段、叶片为外植体进行组培试验。通过在MS培养基上附加不同激素配比,筛选出最佳外植体和最佳培养基配方。[结果]叶芽为最佳诱导外植体。最佳消毒时间组合为75%乙醇10 s与2%Na Cl O 4 min,污染率低至2%。J-12蓝靛果忍冬品种诱导叶芽分化及增殖的最佳培养基及激素配比是MS+NAA0.15 mg/L+6-BA1.50 mg/L,增殖倍数最高可达8.0倍;最佳生根培养基为1/2MS+IBA0.50 mg/L+NAA0.20 mg/L,生根率为100.00%。[结论]该研究可为加拿大蓝靛果忍冬的大规模生产提供技术支持。

[Objective] To study the tissue culture technique of Canada＇s Lonicera edulis.[Method] The leaf buds,flower buds,stem segments and leaves of the fine strain J-12 which were selected by Heilongjiang Forest Botanical Garden were used in tissue culture test. The best explants and best media formulations were selected by adding different hormone ratios on MS medium.[Result]Leaf buds were the best induced explants.The optimal disinfection time combination was 75% alcohol 10 s and 2% Na Cl O 4 min,and the pollution rate was low to 2%. The optimal medium for leaf bud differentiation and proliferation of the J-12 was MS ＋ NAA0. 15 mg/L ＋ 6-BA1. 50 mg/L,with a maximum multiplication factor of up to 8. 0 times. The best rooting medium was 1/2 MS ＋ IBA0. 50 mg/L ＋ NAA 0. 20 mg/L,the rooting rate was 100. 00%.[Conclusion] The study provided technical support for the large-scale production of Canada＇s Lonicera edulis.