不同抗原修复方法对食管鳞癌组织中ANO1蛋白染色的影响

the Effect of Varies Antigen Repairing Methods on the Results of ANO1 Immunohistochemistry Staining in Esophageal Squamous Cell Carcinoma

目的:探讨不同修复条件及修复液对食管鳞癌组织中ANO1的阳性表达程度的影响,筛选优势抗原修复方法。方法:对30例食管鳞癌组织的石蜡切片分别采用高压-枸橼酸缓冲液修复法、高压-EDTA缓冲液修复法、微波-枸橼酸缓冲液修复法及微波-EDTA缓冲液修复法进行修复,检测ANO1蛋白免疫组化染色效果。结果:食管鳞癌组织中ANO1蛋白经高压-枸橼酸缓冲液修复后,着色强度较其他修复方法明显增强,且阳性定位准确、对比度清晰,极大降低免疫组化背景着色。结论:在ANO1蛋白免疫组织化学染色中,高压-枸橼酸缓冲液修复法优于其他抗原修复方法。

Objective： To explore the effect of different antigen repairing condition and repair liquid on degree of ANO1 positive expression in esophageal squamous cell carcinoma, thereby screening advantage antigen repair methods. Methods： The 30 cases of esophageal squamous cell carcinoma tissue paraffin section were respectively examined the immunohistochemical staining effects of ANO1 by high pressure-citrate buffer repair method, high pressure-EDTA buffer repair method, microwave-citrate buffer repair method and microwave-EDTA buffer repair method. Results： ANO1 protein in esophageal squamous cell carcinoma tissues was repaired by high pressure-citrate buffer, tinting strength enhanced obviously than other repair methods, and positive for accurate positioning, clear contrast, greatly reduce the background of immunohistochemical staining.Conclusion：The high pressure-citrate buffer repair method is superior to the other antigen repair methods in immunohistochemical staining of ANO1 protein.