亲和色谱技术在药物分析中的应用进展

Progress in application of affinity chromatography inpharmaceutical analysis

亲和色谱是依靠键合在固定相上的配位体与生物活性目标分子间特异性的识别与可逆的亲和作用,实现生物分子选择性分离的一种液相色谱分析方法。该方法具有高选择性、高回收率等特点,可同时进行色谱分离及活性筛选,广泛应用于活性产物的筛选、分离和纯化。本文简述了生物特效亲和色谱、印迹分子亲和色谱、染料配基亲和色谱等几种常用亲和色谱技术,综述了近年来亲和色谱在手性药物拆分、天然药物中活性组分筛选、活性蛋白分离纯化及药物-蛋白相互作用研究中的应用,并对其应用前景进行了展望。

Affinity chromatography（AC）is a type of liquid chromatography that makes use of biological-like interactions for separation and specific analysis of bioactive components.It has been widely used as a highthroughput screening method for the separation,screening and purification of the target molecules from complex samples with advantages such as high selectivity and high recovery efficiency.This article summarizes the biological effects of affinity chromatography, molecular imprinting chromatography, and dye ligands affinity chromatography.The review also encompasses the application of AC in the separation of chiral drugs,screening of active components,purification of target protein,and mechanism of the drug-protein interaction.Moreover,the prospects of its applications are also discussed.