葡萄柚茎尖微芽嫁接成活率的研究

Using Puncture Method to Improve the Survival Rate of Shoot-tip Micrografting Technology

以曼赛龙柚为砧木,不同品种葡萄柚的成年态茎段为外植体,不同植物生长调节剂浓度对茎段诱导产生的不定芽作为接穗,通过不同嫁接方法、接穗品种及来源对微芽嫁接成活率的影响试验,改进微芽嫁接方式,提高嫁接苗的成活率。结果表明:不定芽诱导的最佳培养基为WPM+6-BA 1.0 mg/L+NAA 0.2 mg/L+GA 0.2 mg/L;穿刺法更适合葡萄柚微芽嫁接,能显著提高嫁接成活率;不同接穗品种葡萄柚微芽嫁接成活差异较大,5个接穗品种微芽嫁接成活率依次为:瑞路比>星路比>哈路比>火焰>里约红。试管诱导不定芽作为接穗,嫁接成活率76.67%;田间嫩梢接穗的茎尖微芽嫁接成活率仅为38.33%,试管诱导接穗成活率显著提高。

Experiment with Citrus maxima cv. Mansailong as root stock, the adult stem segments of different C. paradisi cuhivars were used as explants to induce adventitious buds, which could improve the survival rate of grafted seedlings. Adventitious buds induced by stem segments at different concentrations of plant growth regulator were used as scions, the effect of different grafting methods, scion varieties and sources on the survival rate of shoottip micrografting was studied. The results showed that the best medium for adventitious bud induction is WPM ＋ 6- BA 1.0 mg/L ＋ NAA0. 2 mg/L ＋ GA 0. 2 mg/L; it was found that the puncture method was more suitable for C. paradisi shoot-tip micrografting and it can significantly improve the survival rate of grafting; Shoot-tip micrografting has a significant differences in the graft survival rate of different scion varieties C. paradisi, the survival rate was Ray Ruby 〉 Star ruby 〉 Hudson Foster 〉 Flame 〉 Rio Red; Induction of adventitious bud of graft survival rate was 76.67%, field experiment graft survival rate is 38.33%.