烤烟‘K326’抗TMV防御物质初探

Anti-TMV activity of the extracts from tobacco‘K326'

烤烟主栽品种‘K326’在打顶后,随着次生代谢产物的积累,烟草花叶病症状呈减轻趋势。本试验利用半叶枯斑法对‘K326’的全株、根、茎、叶甲醇提取物及根部不同极性溶剂萃取物进行抗烟草普通花叶病毒活性筛选,结果表明:在浓度为1mg/mL时,‘K326’各部位甲醇提取物中,根部甲醇提取物的活性最强,对TMV体外钝化、初侵染和复制增殖作用的抑制率分别为74.50%、68.82%和47.76%;利用不同极性溶剂对根部甲醇提取物萃取处理后,乙酸乙酯萃取物活性增强明显,对TMV体外钝化、初侵染和复制增殖作用的抑制率分别为76.81%、78.02%、61.42%;经普通烟‘K326’进一步验证,根部甲醇提取物的乙酸乙酯萃取物活性最强。综上,‘K326’中抗病毒防御物质可能存在于根部甲醇提取物的乙酸乙酯萃取物中。

The antiviral activity of the extract from‘K326＇against Tobacco mosaic virus（TMV）was determined by a half-leaf necrosis method.The methanol extracts from different parts of‘K326＇showed good inhibitory activities against TMV at 1 mg/mL.Especially the methanol extracts from roots exhibited the best inhibitory activities on TMV inactivation,primary infection,and proliferation with an inhibition rate of 74.50%,68.82% and47.76%,respectively;the methanol extracts from roots were disposed with different organic solutions,i.e.,petroleum ether,ethyl acetate and n-butanol;after that,the antiviral activity of the extracts with ethyl acetate increased significantly,with an inhibition rate of 76.81%,78.02% and61.42%,respectively.Further experiments on‘K326＇suggested that the antiviral activity of the extracts of ethyl acetate was the best.