血管紧张素原基因T704C和C521T多态性与替米沙坦降压疗效关系研究

Relationships between T704C and C521T polymorphisms of angiotensinogen gene and antihypertensive responses to telmisartan in hypertension patients

目的探讨血管紧张素原基因T704C和C521T多态性与替米沙坦降压疗效之间的关系。方法选择符合WHO/ISH高血压诊断标准的轻中度高血压患者148例,给予替米沙坦治疗8周,在观察疗效的同时,应用聚合酶链反应(PCR)对患者AGT基因T704C和C521T多态性进行分析,同时测定空腹血糖、血脂、肝功能、肾功能、血电解质、AngⅡ水平。结果治疗前后BMI、心率、肝肾功能、血电解质比较差异均无统计学意义(P均>0.05),AngⅡ水平明显增高(P<0.05)。药物治疗后AGT基因T704C和C521T位点每一组患者的SBP、DBP均较治疗前明显降低(P均<0.05),但2个SNPs位点间治疗前后SBP、DBP及降压幅度比较差异均无统计学意义(P均>0.05)。结论 AGT基因T704C和C521T多态性不能预测替米沙坦降压疗效。

Objective It is to investigate whether T704C and C521T polymorphisms of angiotensinogen gene were associated with blood pressure（BP） response to angiotensin II receptor blocker （ARB） in hypertensive in China. Methods 148 patients with mild-to-moderate primary hypertension according with the diagnosis standard of WHO/ISH hypertension were selected and given monotherapy with telmisartan and then were followed up for 8 weeks. The T704C and C521T polymorphisms of the AGT gene were determined through polymerase chain reaction and restriction fragment-length polymorphism. The relationship between these polymorphisms and the changes in BP was analyzed while the curative effect was evaluated. The levels of FPG, blood liquid, renal and liver function, blood electrolytes, ang Ⅱ were detected at the same time. Results Compared with that before treatment, the difference in BMI, HR, liver and kidney function, blood electrolytes were not significant after treatment （P 〉 0. 05）, but the level of Ang Ⅱ was increased obviously （P 〈 0.05）. After medical treatment, SBP and DBP was decreased significantly in each patient with AGT gene T704C and C521T compared with that before treatment（ P 〈 0. 05 ）, but there was no significant difference in SBP and DBP before and after treatment and reduction of BP between 2 SNPs （P 〉 0. 05 ）. Conclusion AGT gene T-/04C and C521T polymorphisms is not associated with BP response to telmisartan treatment in hypertensive patients.