宫颈癌患者肿瘤组织中弗林蛋白酶的表达与临床病理因素及局部免疫功能的关系

Association of Furin protease expression in tumor tissue with clinicopathological factors and local immune function in cervical cancer patients

目的观察宫颈癌患者肿瘤组织中弗林蛋白酶的表达与肿瘤浸润、T淋巴细胞亚群的关系。方法选取2014年1月至2015年12月收集的56例宫颈癌患者作为研究对象,检测患者肿瘤组织中弗林蛋白酶表达水平,并计数局部免疫细胞T淋巴细胞亚群:CD8^+、CD4^+、CD4^+/CD8^+、颗粒酶B(granzyme B)^+、CD134^+及叉头翼状螺旋转录因子(forkhead box protein P3,FOXP3)^+细胞。采用Spearman相关分析分析肿瘤组织中弗林蛋白酶表达水平与患者临床病理因素及肿瘤组织中CD8^+、CD4^+、CD4^+/CD8^+、颗粒酶B^+、CD134^+及FOXP3^+细胞计数的相关性。结果患者年龄、肿瘤分化程度与弗林蛋白酶表达水平无显著相关性(P均>0.05),国际妇产科联盟(FIGO)宫颈癌分期、浸润深度及淋巴结转移与弗林蛋白酶表达水平具有显著相关性(P均<0.05)。弗林蛋白酶表达水平与CD4^+细胞计数、CD4^+/CD8^+及FOXP3^+细胞计数呈显著正相关(P均<0.05),与CD8^+细胞、颗粒酶B^+细胞、CD134^+细胞细胞计数呈显著负相关(P均<0.05)。结论宫颈癌患者肿瘤组织弗林蛋白酶表达水平与肿瘤分期、浸润深度、淋巴结转移及局部免疫功能具有相关性。

Objective To investigate the relationship between Furin protease （Furin）expression in tumor tissues and tumor infiltrating,local immunocytes T-lymphocyte subsets in patients with cervical carcinoma. Methods A total of 56 patients with cervical carcinoma were selected as study objects from January 2014 to December 2015 to detect Furin expression level in tumor tissues and take count the local immunocyte, T-lymphocyte subsets CD8 ＋ , CD4 ＋ , CD4 ＋/CD8 ＋ , Granzyme B＋ ,CD134 ＋ and FOXP3 ＋ cells. Spearman correlation analysis was used to analyze the correlations of Furin expression levels with clinicopathological factors and cell counts of CD8 ＋ , CD4 ＋ , CD4 ＋/CD8 ＋ , Granzyme B ＋ , CD134 ＋ and FOXP3 ＋ cells. Results Furin expression level was not significantly correlated with patients＇ age and tumor differentiation degree （ all P 〉 0. 05 ） , but was significantly positively correlated with the staging of cervical cancer by International Federation of Gynaecology and Obstetrics （ FIGO）, tumor invasion depth and lymph node metastasis （ all P 〈0. 05 ）. Moreover, Furin expression level was significantly positively correlated with CD4 ＋ , CD4 ＋/CD8 ＋ and FOXP3 ＋ cells counts（all P 〈 0. 05 ）, and was significantly negatively correlated with CD8 ＋, Granzyme B ＋ and CD134 ＋ cell counts（ all P 〈 0. 05）. Conclusion Furin expression level in tumor tissues is associated with tumor staging, tumor invasion depth, lymph node metastasis and local immune functions in cervical carcinoma patients.