诱导型一氧化氮合酶和缺氧诱导因子-1α与尖锐湿疣发生、发展的相关性研究

Effect of iNOS and HIF-1α on condyloma acuminatum

目的检测尖锐湿疣(CA)组织中诱导型一氧化氮合酶(iNOS)与缺氧诱导因子-1α(HIF-1α)的表达,以明确其在CA发生、发展中的临床意义。方法收集50例CA和20例正常包皮组织,采用免疫组织化学法检测组织中iNOS与HIF-1α的表达并与CD34表达进行相关分析。结果 50例CA患者中,iNOS阳性50例(100%),表现为表皮全层细胞均为iNOS阳性表达;对照组20例正常皮肤中iNOS阳性14例(70%),iNOS阳性表达主要位于表皮基底层,且呈弱阳性表达,CA患者皮损中iNOS灰度值为(97.985±20.320)高于对照组(73.017±15.633)(P<0.05)。50例CA患者中42例HIF-1α阳性(84%),高于对照组阳性9例(45%)(P<0.05);且HIF-1α在CA患者中灰度值为(0.204±0.064),高于对照组(0.135±0.019)(P<0.05)。CA患者组织中iNOS与CD34表达呈正相关(r=0.375,P=0.007);CA患者组织中HIF-1α与CD34表达呈正相关(r=0.393,P=0.005)。结论 iNOS与HIF-1α可能协同参与CA的发病和发展。

Objective To investigate the expression of inducible nitric oxide synthase （iNOS） and hypoxiainducible factor-1α （HIF-1α） and their effect on development of condyloma acuminatum （CA）. Methods Atotal of 50 patients with CA and 20 healthy volunteers were collected. The expression of iNOS, HIF-1α andCD34 was determined by Immunohistochemistry. Results A total of 50 cases with CA were identified as iNOSpositive （100%） which were expressed in whole epidermis; 14 out of 20 volunteers （70%） were iNOS positive（weak signal） which were mainly expressed in epidermal basal cells. Gray value of iNOS in CA patients washigher than that in healthy volunteers （97.9854 依 20.3196 vs 73.0174 依 15.6330, respectively）（ 〈 0.05）. Forty-two out of 50 patients （84%） experienced increased expression of HIF -1琢 while only9 volunteers hadpositive signals （45%） （ 〈 0.05）. Gray value of HIF-1α in CA patients was higher than that in the controlgroup （0.2035 ± 0.0637 vs 0.1349 ± 0.0194） （ 〈 0.05）. Intimate correlation between iNOS or HIF-1α andCD34 in CA was observed （ = 0.375 and 0.393, 〈 0.05）. Conclusion iNOS and HIF-1α is involved in thepathogenesis of CA.