3种血清分离法及血清存放时间对感染检测结果的影响

Effect Evaluation of Three Equine Serum Preparation Protocols with Different Separation Methods and Storage Time on Detection of Toxoplasma gondii Infection

[目的]以检测马弓形虫感染为例,观察4℃冰箱过夜、37℃水浴锅法及室温放置3种血清分离法及血清存放时间对检测结果的影响。[方法]自然凝固后的马血液,分别在4℃、室温、37℃下析出血清,用间接血凝试验(IHA)检测血清中的弓形虫抗体;对检测出的阳性血清标本分别放置在4℃、室温下,15 d内每天检测其抗体效价。[结果]3种方法分离出的血清,在血清量、血清颜色、血清清亮度方面差异不明显,IHA检测结果一致;4℃、室温2种温度下放置的阳性血清标本,15 d内血清的清亮度无改变,抗体效价无改变。[结论]4℃冰箱过夜、37℃水浴锅法及室温放置3种方法分离出的血清,均可用于马弓形虫感染的检测;4℃、室温下存放15 d内的血清仍可用于检测。

[Objective]The detection effect of equine Toxoplasma gondii infection with serum samples prepared by three different protocols, including 4 ℃ storage overnight, 37 ℃ incubation in water bath, and stationary incubation at room temperature, was assessed in this study. [Methods]The horse blood samples after natural coagulation were placed at 4 ℃, room temperature, and37 ℃ respectively, and the obtained serum samples were subjected to Toxoplasma gondii antibody detection by IHA method; the antibody positive serum samples were selected and placed at 4 ℃ and room temperature respectively, and the antibody titer were determined daily for fifteen consecutive days. [Results]No differences in volume, color and brightness of the serum samples prepared with the above three protocols were observed; the same results were obtained in Toxoplasma gondii antibody detection for the serum samples prepared by different protocols; during the fifteen days observation duration, the brightness and antibody titer of the antibody positive serum samples placed at both 4 ℃ and room temperature had no obvious change. [Conclusion]The serum samples prepared by 4 ℃ storage overnight, 37 ℃ incubation in water bath and stationary incubation at room temperature are all qualified to be used in detection of Toxoplasma gondii infection; serum samples storage at 4 ℃ and room temperature for fifteen days are still able to be used for Toxoplasma gondii antibody detection.