2017年内蒙古部分地区奶牛乳房炎金黄色葡萄球菌分离鉴定及耐药性分析

Isolation, Identification and Antimicrobial Resistance Profiles of Staphylococcus aureus from Dairy Cows with Mastitis in Selected Regions of Inner Mongolia in 2017

采集内蒙古部分地区(呼和浩特市、包头市)乳房炎患病牛乳样50份,进行金黄色葡萄球菌的分离鉴定及药敏试验。经分离鉴定,共检出8株金黄色葡萄球菌,分离率为16.0%。药敏试验结果显示,8株金黄色葡萄球菌对环丙沙星12.5%耐药,对氧氟沙星75.0%耐药,对氨曲南和青霉素100%耐药,对红霉素37.5%耐药,对氨苄西林37.5%耐药;对利福平、复方新诺明、氯霉素、万古霉素、头孢西丁、庆大霉素、四环素、克林霉素、苯唑西林、妥布霉素、利奈唑胺、美罗培南、阿莫西林—克拉维酸100%敏感。

A total of fifty quarter milk samples were collected from dairy cows with mastitis in selected regions of Inner Mongolia（Hohhot and Baotou City）. Standard microbiological methods were used to isolate Staphylococcus aureus（S. aureus） strains and the suspicious isolates were subsequently phenotypically and molecularly identified. Antimicrobial resistance profiles of the isolates were assessed. Eight S. aureus isolates were obtained from the collected samples and the isolating rate was 16.0%; the antimicrobial susceptibility test results revealed that 12.5% of the isolates were resistant to ciprofloxacin, 37.5% of the isolates showed resistance to erythromycin and ampicillin, 75.0% of the isolates exhibited resistance to ofloxacin, and 100% of the isolates were resistant to aztreonam and penicillin. No resistance was detected for rifampicin, compound sulfamethoxazole,chloramphenicol, vancomycin, cefoxitin, gentamicin, tetracycline, clindamycin, oxacillin, tobramycin, linezolid, meropenem, and amoxicillin/clavulanate.