IL-9、IL-10在免疫性肝损伤中的作用及临床意义

Study on role and clinical significance of IL-9,IL-10 in immune liver damage

目的初步探讨几类免疫性肝损伤患者血清中白细胞介素(IL)-9和IL-10的表达水平及临床意义。方法选取2016年1-6月于该院确诊的慢性乙型肝炎患者(慢性乙肝组)20例,自身免疫性肝炎患者(自身免疫性肝炎组)20例,药物性肝炎患者(药物性肝炎组)20例,同时选取体检健康者(对照组)20例,用酶联免疫吸附试验分别检测外周血中IL-9和IL-10的水平,实时荧光定量聚合酶链反应检测IL-9、IL-10的mRNA表达水平,组间及组内数据进行对比和相关性分析。结果与对照组相比,慢性乙肝组、自身免疫性肝炎组及药物性肝炎组的IL-9表达明显增高,IL-10均有不同程度减低,差异有统计学意义(P<0.05)。结论 IL-9可能在免疫性肝病中正向调节免疫应答过程,促进免疫效应细胞对肝脏组织的破坏作用,而IL-10在肝损伤中表达下降提示其可能抑制免疫细胞对肝细胞的破坏作用,IL-9与IL-10水平监测可在临床免疫性肝病早期诊断及治疗监测中发挥一定作用。

Objective To preliminarily investigate the expression level and clinical significance of serum interleukin（IL）-9and IL-10 in the patients with several immunological liver injury.Methods Twenty cases of 20 chronic hepatitis B（chronic hepatitis B group）,20 cases of autoimmune hepatitis（autoimmune hepatitis group）and 20 cases of drug-induced hepatitis（drug-induced hepatitis group）in the hospital from January to June 2016 were selected,meanwhile 20 healthy persons by physical examination were selected as the control group.The contents of IL-9and IL-10 were detected by using enzyme-linked immuno sorkent assay.The realtime fluorescent quantitative polymerase chain reaction was used to detect the expression levels of IL-9and IL-10 mRNA.The intragroup and inter-group data were performed the comparative and correlation analysis.Results Compared with the healthy control group,the IL-9expression level in the hepatitis Bgroup,autoimmune hepatitis group and drug-induced hepatitis group was significantly increased,while the IL-10 level had difference degrees of decrease,the difference was statistically significant（P〈0.05）.Conclusion IL-9can positively adjust the immune response process in the autoimmune liver disease,promotes the damage effect of immune effector cells on the liver tissue,while the IL-10 expression decrease in liver injury indicates that it could inhibit the damage role of immune cells on liver cells.The monitoring of IL-9and IL-10 contents can play a certain role in early diagnosis and treatment of immune liver disease.