摘要
目的观察肝癌患者血清和组织高迁移率族蛋白B1(HMGB1)水平的变化,探讨HMGB1在肝癌发生、发展中的作用。方法应用酶联免疫吸附试验和免疫印迹试验定量检测40例对照组、38例良性肝病组患者及40例肝癌组患者的术前血清和组织HMGB1水平,并分析其与临床病理分期、肿瘤大小、分化程度以及淋巴结转移之间的关系。结果肝癌患者血清HMGB1水平明显高于对照组,差异有统计学意义(P<0.01);且血清HMGB1水平与TNM分期、分化程度及淋巴结转移密切相关,差异有统计学意义(P<0.01);与肿瘤大小无关,差异无统计学意义(P>0.05);HMGB1在肝癌组织中的相对表达值明显高于癌旁组织和健康肝脏组织。结论HMGB1水平在肝癌患者血清中明显升高,同时HMGB1在肝癌组织中高表达,提示HMGB1检测对肝癌的早期诊断有重要价值。
Objective To investigate the change of serum and tissue high mobility group box 1（HMGB1）levels in the patients with hepatic carcinoma and its role in the occurrence and progress of hepatic carcinoma.Methods The enzyme-linked immunosorbent assay and Western-blot were used to measure the levels of liver tissue and serum HMGB1 in 40cases of hepatic carcinoma group,38 cases of benign liver diseases group and 40 controls group.Then their relation with clinicopathologic stage,tumor size,differentiation degree and lymph node metastasis was analyzed.Results Serum HMGB1 level in hepatic carcinoma patients was significantly higher than that in healthy controls,and the difference was statistically significant（P〈0.01）,moreover the serum HMGB1 level was closely related with the TNM stage,tumor differentiation degree and lymph node metastasis,the difference was statistically significant（P〈0.01）,however,had no relation with the tumor size,the difference was not statistically significant（P〉0.05）.The relative expression of HMGB1 in liver carcinoma tissue was significantly higher than that in the paracancerous tissue and healthy liver tissue.Conclusion The level of serum HMGB1 is increased in in the patients with hepatic carcinoma,meanwhile HMGB1 is highly expressed in liver carcinoma tissue,prompting that the HMGB1 determination has an important value in early diagnosis of hepatic carcinoma.
出处
《检验医学与临床》
CAS
2017年第20期3015-3017,3020,共4页
Laboratory Medicine and Clinic
