滇细辛水提取物小鼠灌胃给药毒性和体内抗炎活性研究

Study on Acute Toxicity in Mice and in Vivo Anti-inflammatory Activity of Aqueous Extract from Asarum Delavayi Franch

目的考查滇细辛水提取物小鼠灌胃给药急性毒性和体内抗炎作用。方法采用近似最小致死量联合最大耐受量法测定小鼠灌胃给药急性毒性反应;采用巴豆油致小鼠耳廓肿胀实验、角叉菜胶致大鼠足趾肿胀实验、以及大鼠植入性棉球肉芽生成实验,评价滇细辛水提取物体内抗炎效应。结果小鼠急性毒性实验结果表明:(1)滇细辛水提取物小鼠灌胃给药最小致死量为15.12g/kg(折算为77.62g生药/kg),最大耐受量为13.62g/kg(折算为69.92g生药/kg);(2)给药后3、7、14d,与溶媒组比较,滇细辛水提取物各给药组动物体重无明显差异;(3)实验结束后解剖发现,滇细辛水提取物各给药组部分动物肾脏颜色变浅。体内抗炎实验结果表明:(1)与溶媒组比较,滇细辛(0.84g/kg,折算为4.31g生药/kg)明显降低巴豆油致炎小鼠耳廓肿胀度。(2)角叉菜胶致炎60、120、240、360min时,滇细辛(0.84g/kg,折算为4.31g生药/kg)明显降低角叉菜胶致炎大鼠足趾肿胀度。(3)与溶媒组比较,滇细辛(0.42、0.84g/kg,折算为2.16g生药/kg、4.31g生药/kg)对大鼠植入性棉球肉芽重量无明显影响。结论滇细辛水提取物存在一定毒性,且其主要毒性靶器官为肾脏。滇细辛水提取物对巴豆油所致小鼠耳廓肿胀、角叉菜胶致大鼠足趾肿胀等急性、亚急性炎症具有明显抑制作用,而对大鼠植入性棉球肉芽所致慢性炎症无明显抑制作用。

Objective To investigate acute toxicity in mice and in vivo anti - inflammatory activity of aqueous extract from delavayi Franch. Methods The acute toxicity of the extracts in mice was determined by minimum lethal dose （MLD） combined with maximum tolerance dose （MTD）. Croton oil - induced mouse ear edema, carrageenan -induced rat toe edema, and cotton pellet granulation tissue hyperplasia were used to assess the in vivo anti - inflammatory effect of the extract. Results The acute toxicity results showed that ! the MLD of the aqueous extract from delavayi Franch was 15.12 g/kg and the MTD was 13.62g/kg.② 3, 7,and 14 days after the administrations, there were no significant differences in the body weight among the vehicle group and various dose groups of the extract.③ At the end of the experiment, the anatomical results suggested that the color of the kidney became lighter in parts of the animals after the administrations. The in vivo anti - inflammation results revealed that ! compared with the vehicle group, the aqueous extract（0.84g/kg, equal to 4.31g crude drug/kg） markedly reduced the ear edema of the mice.② the extract（0.84g/kg, equal to 4.31g crude drug/kg） remarkably decreased carrageenan - induced rats toe swelling 60, 120, 240, 360 min after the sensitization.③ the extract （0.42、0.84g/kg） had no significant inhibitory effect on the cotton pellet granulation tissue hyperplasia. Conclusion The aqueous extract from As a r u m d e la v a y i Franch has a certain toxicity, and its toxic target organs are kidneys. The extract significantly suppresses acute inflammation such as croton oil -induced mouse ear edema and carrageenan -induced rat toe edema and not chronic inflammation like cotton pellet granulation tissue hyperplasia.