摘要
为了解抗生素菌渣堆肥中抗生素残留对抗生素抗性基因(ARGs)环境行为的影响,以青霉素菌渣堆肥为对象,采用实时定量PCR方法分析了8种典型β-内酰胺酶基因,bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-IMP-1)、bla_(-VIM-2)、bla_(-CMY)、bla_(-OXA-23)、bla_(-NDM-1)在整个堆肥过程中的丰度变化.结果表明,高温堆肥处理大大缩短了青霉素的降解时间;bla-NDM-1在所有样品中均未检出.通过比较β-内酰胺酶基因在不同处理中第1d和30d的绝对数量变化,在处理组中除bla_(-IMP-1)、bla_(-VIM-2)基因绝对数量有所增加外;其他基因都明显减少.从相对丰度看,在堆肥前期,青霉素残留对bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-CMY)、bla_(-OXA-23)、bla_(-VIM-2)基因有一定诱导富集效应.随着堆肥进程及菌渣堆肥中抗生素浓度的降低,到了堆肥末期,各处理组及对照组bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-CMY)的相对丰度较堆肥前期显著降低;而处理组中bla_(-IMP-1)、bla_(-VIM-2)的相对丰度较堆肥前期显著增加.
In order to reveal the environmental impacts of antibiotic resistance genes(ARGs) during penicillin biomass-residue composting because of the possible antibiotic residues, the relative abundances and distributions of eight typical β-lactamase genes(bla-TEM、bla(-CTX-M-1)、bla(-CTX-M-9)、bla(-IMP-1)、bla(-VIM-2)、bla(-CMY)、bla(-OXA-23)、bla(-NDM-1)) were investigated with quantitative PCR technique. The results indicated that high temperature composting greatly shortened the degradation time of penicillin. No bla(-NDM-1) gene was detected in any sample. The abundances of all studied genes were significantly reduced from day 1 to day 30 in the different penicillin biomass-residue composting experiments except those of the bla(-IMP-1)and bla(-VIM-2) genes which slight increased. The penicillin residue induced the increases of relative abundance of bla-TEM, bla(-CTX-M-1). bla(-CTX-M-9), bla-CMY, bla(-OXA-23), and bla(-VIM-2) genes during the early composting stage. With the elongation of the composting process, penicillin residue gradually degraded. At the end of composting, the relative abundances of bla-TEM、bla(-CTX-M-1)、bla(-CTX-M-9)、bla-CMY significantly decreased in all treated samples and the control in comparison with those of bla(-IMP-1)、bla(-VIM-2), which greatly increased.
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2017年第10期3873-3881,共9页
China Environmental Science
基金
国家自然青年科学基金资助项目(41401363
41301278)
江苏省自然青年基金资助项目(BK20130102)
环境保护部环保公益性行业科研专项(201209024)
2016年公益性科研院所基本科研业务费项目