毛细支气管炎患儿检测外周血CXCR3及其配体的临床意义

Clinical Significance of Detecting CXCR3 and Its Ligand in Peripheral Blood of Children With Bronchiolitis

目的探讨毛细支气管炎患儿检测外周血CXCR3及其配体Mig、IP-10、I-TAC的临床意义。方法选择2016年6月—2017年5月在我院儿科诊断治疗的毛细支气管炎患儿60例为研究对象,另选择同期门诊健康体检的一般资料匹配的婴幼儿30例为对照组。毛细支气管炎患儿60例根据是否有过敏因素分为过敏亚组30例与无过敏亚组30例。外周血CXCR3检测采用流式细胞术,血清Mig、IP-10、I-TAC水平检测采用ELISA方法。结果毛细支气管炎患儿无论是否有过敏,CD4+CD183+/CD4+、CD8+CD183+/CD4+均高于对照组,差异有统计学意义(P<0.05);过敏亚组高于无过敏亚组,差异有统计学意义(P<0.05)。毛细支气管炎患儿无论是否有过敏,外周血清Mig、IP-10、I-TAC水平均高于对照组,差异有统计学意义(P<0.05);过敏亚组I-TAC水平高于无过敏亚组,差异有统计学意义(P<0.05)。结论毛细支气管炎患儿检测外周血CXCR3及其配体水平升高,尤其有过敏因素的患儿升高更明显。

Objective To discuss the clinical significance of detecting CXCR3 and its ligand in peripheral blood of children with bronchiolitis. Methods 60 cases with bronchiolitis from June 2016 to May 2017 were selected as objects, and 30 cases of matched infants were selected as control group. 60 cases with bronchiolitis were divided into 30 cases in allergic group and 30 cases in no allergic group according to whether with allergic factors. Peripheral blood CXCR3 was detected by flow cytometry, and serum Mig, IP-10 and I-TAC levels were detected by ELISA. Results Children with bronchiolitis, whether or not they had allergies, CD4＋ CD183＋ / CD4＋ 、CD8＋ CD183＋ / CD4＋ were higher than those in the control group（P 〈 0.05）; The allergic group was significantly higher than the no allergic subgroup, which showed significant difference（P 〈 0.05）. Children with bronchiolitis, whether or not they had allergies, peripheral serum Mig, IP-10, I-TAC were higher than those in the control group（P 〈 0.05）; I-TAC in the allergic group was significantly higher than the no allergic subgroup, which showed significant difference（P 〈 0.05）. Conclusion The levels of CXCR3 and its ligands in peripheral blood of children with capillary bronchitis are significantly increased, especially in children with allergic factors.