残留型精神分裂症患者肠道菌群特点初探

A preliminary study of intestinal flora in patients with residual schizophrenia

目的:初探残留型精神分裂症患者的肠道菌群特点。方法:选取20名残留型精神分裂症患者作为观察组,20名健康人作为对照组,对两组人群的粪便样本进行检测。通过"各个样品的总基因组DNA提取"、"16S r DNA的荧光PCR扩增"、"PCR扩增产物纯化"、"PCR纯化产物酶切"、"酶切产物脱盐"、"电泳进样体系和电泳参数"、"T-RFLP图谱生成"和"T-RFLP数据处理"等7个步骤而得出相应的结果。结果:两组的肠道微生物群落之间存在明显的差异。结论:残留型精神分裂症的肠道菌群与健康人存在差异。

Objective： To preliminarily investigate characteristics of intestinal flora of patients with residual schizophrenia.Methods： A total of 20 patients with residual schizophrenia were selected as the observation group,and 20 healthy subjects were used as the control group.Their fecal samples were collected for the study.The corresponding results were got through 7 steps,including total genomic DNA extractionofvarious samples,16 S rDNA fluorescent PCR amplification,purification of the PCR amplified products,PCR purified product enzyme digestion,desalting of the enzyme digested products,electrophoresis sample system and electrophoresis parameters,T-RFLP map generation and T-RFLP data processing.Results： There was a marked difference between the intestinal micro-biota between the observation group and the control group.Conclusions： The intestinal flora of the residual schizophrenics is different from that of the healthy people.