五倍子对白色念珠菌生物被膜的抑制作用

Inhibitory effect of Gallnut on Candida albicans biofilm

目的研究五倍子对白色念珠菌菌株生物被膜的抑制作用。方法采用MTT法测定五倍子对生物被膜的形成及成熟生物被膜的抑制作用。采用RT-PCR法测定五倍子对ALS1和CPH1基因表达量的影响。结果五倍子能显著抑制白色念珠菌生物被膜的形成,其抑制生物被膜形成的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）均为128 mg/mL。五倍子对白色念珠菌的成熟生物被膜也有较强的抑制作用,其抑制成熟生物被膜的MIC为256 mg/mL。五倍子能显著降低白色念珠菌ALS1和CPH1的基因表达量,其中64 mg/mL的五倍子作用白色念珠菌16 h后,与对照组相比,ALS1和CPH1的基因表达量分别降低了79.2%和82.0%。结论五倍子对白色念珠菌生物被膜的形成和成熟生物被膜均具有显著的抑制作用,其作用机制可能与降低ALS1和CPH1的基因表达量,抑制白色念珠菌的粘附和侵袭有关。

Objective To study the inhibitory effect of Gallnut on the biofilm of Candida albicans.Methods MTT assay was used to determine the inhibitory effect of Gallnut on biofilm formation and mature biofilm.RT-PCR was used to determine the effect of Gallnut on the expressions of ALS1 and CPH1 genes.Results Gallnut has a stronger inhibitory effect on Candida albicans biofilm formation,the minimum inhibitory concentration（MIC） and the minimum fungicidal concentration（MFC） for inhibition of biofilm formation both are 128 mg/mL.Gallnut also has a strong inhibitory effect on the mature biofilm of Candida albicans,the minimum inhibitory concentration（MIC） is 256 mg/mL.Gallnut could significantly reduce the gene expressions of ALS1 and CPH1.Compared with the control group,64 mg/mL Gallnut act on Candida albicans after 16 h,the gene expression of ALS1 and CPH1 decreased by 79.2% and 82.0% respectively.Conclusion Gallnut has a significant inhibitory effect on the formation and maturation of Candida albicans biofilm.The inhibitory effect of Gallnut on the adherence and invasion of Candida albicans is mainly through inhibiting the expressions of ALS1 and CPH1 genes.