辛通畅络中药复方对大鼠血管平滑肌细胞迁移的影响

Effect of Chinese herbal medicine of Xintong Changluo on the migration of vascular smooth muscle cells in rats

[目的]观察辛通畅络中药复方对血管平滑肌细胞(VSMCs)迁移的影响。[方法]以含辛通畅络中药处方不同浓度(10%、20%、30%)的大鼠血清培养VSMCs,以正常大鼠空白血清为对照,采用Boyden小室法及划痕实验观察辛通畅络中药血清对VSMCs迁移的影响;采用Western blot检测MMP-2、MMP-9蛋白表达情况。[结果]辛通畅络中药复方含药血清可以抑制VSMCs的迁移及MMP-2、MMP-9蛋白的表达,并且这种抑制效应呈浓度依赖性。[结论]辛通畅络中药复方具有抑制VSMCs迁移的作用,其作用机制可能与影响MMP-2和MMP-9的表达有关。

[Objective] To observe the effect of Chinese herbal medicine of Xintong Changluo on the migration of vascular smooth muscle cells （ VSMCs）. [Methods] VSMCs was cultured in serum of rats with different concentrations of Xintong Changluo （10%, 20%, 30%）, normal rats’ serum was used as control group. The VSMCs migration was observed by scratch test and Boyden chamber method. The expression of MMP-2 and MMP-9 protein was detected by Western blot. [Results] Serum containing Xintong Changluo can inhibit the migration of VSMCs and the expression of MMP-2 and MMP-9 proteins, and the inhibitory effect was concentration dependent. [Conclusions] Xintong Changluo can inhibit the migration of VSMCs, and the mechanism may be related to its influence on the expression of MMP-2 and MMP-9.