尿酸酶自组装空心纳米微球体外稳定性

In Vitro Stability of Uricase Self-Assembly Hollow Nanospheres

制备载尿酸酶(Uricase,UOX)聚乙二醇-透明质酸(Hyaluronic acid-graft-poly(ethylene glycol),HA-g-PEG)/羟丙基-β-环糊精(Hydroxypropyl-beta-cyclodextrin,HPCD)自组装空心纳米微球(HA-g-PEG/HPCD self-assembly hollow spheres encapsulated uricase,UHPHD),并研究其体外稳定性。制备UHPHD,并测定其包封率、粒径及Zeta电位。再分别从最适温度、最适pH、热稳定性、贮存稳定性、酸碱稳定性和抗胰蛋白酶水解能力初步考察游离UOX和UHPHD的差异。结果:UHPHD的包封率为(62.17±2.94)%,粒径和Zeta电位分别为(299.60±13.05)nm和(-45.10±2.75)mV。UHPHD和UOX最适温度均为40℃,最适pH均为8.5。体外稳定性结果显示,UHPHD的体外稳定性明显高于UOX。

To prepare the self-assembly hollow nanospheres of hyaluronic acid-graft-poly(ethylene glycol)(HA-g-PEG) and hydroxypropyl-beta-cyclodextrin(HPCD) containing uricase(U HPCD)and study their stability in vitro. UHPCD were prepared and the entrapment efficiency,size and zeta potential of UHPCD were detected. The optimum temperature,optimum pH,thermal stability,storage stability,pH stabilities and proteolytic stabilities of UOX and UHPHD were measured. The entrapment efficiency of UHPHD was(62.17±2.94)%. The average particle size was(299.60±13.05)nm,and the zeta potential was(-45.10±2.75) mV. The optimal temperatures for UHPHD and U0 X were 40 ℃,the optimal pH values were 8.5. UHPHD significantly enhanced the stability of free UOX in vitro.