十精丸血清药物化学研究

Preliminary study on serum pharmacochemistry of Shijing pill

目的 利用中药血清药物化学的方法研究十精丸的入血成分.方法 建立口服十精丸后大鼠血清的HPLC指纹图谱,比较十精丸、缺味方、单味药及给药后的血清样品,确定口服十精丸后大鼠血中移行成分及来源生药.结果 十精丸给药后血中出现14个移行成分,4个为血中固有成分,7个为十精丸中原型成分,3个为代谢产物.结论 阐明了十精丸的部分入血移行成分,为进一步研究十精丸的药效物质基础提供参考.

Objective To conduct serum pharmacochemistry study onShijing pill. Methods HPLC fingerprints of serum in rats after takingShijingpill were established,the serun samples after takingShijing pill, decoction without one of the component drugs and single crude drug were compared,the transitional constituents absorbed into the blood and the original crude after takingShijing pill was determined.Results Fourteen transitional constituents were detected in rat blood after takingShijing pill,four of which were permanent component of blood,seven of which were prototype constituents,three of which were metabolites. Conclusions Some of chemical composition ofShijing pill was clarified preliminarily,that contributes to further studying pharmacodynamic meterial foundation ofShijing pill.