摘要
Osteosarcoma is a high-class malignant bone cancer with a less than 20% five-year survival rate due to its early metastasis potential. There is an urgent need to develop a versatile and innoxious drug to treat metastatic osteosarcoma.Curcumin（Cur） has shown its potential for the treatment of many cancers; however,the clinical implication of native curcumin is severely hindered by its intrinsic property. In this study,a mixed system of monomethoxy（polyethylene glycol）-poly（d,l-lactide-co-glycolide）/poly（ε-caprolactone）（m PEGPLGA/PCL） was used to build a formulation of curcuminencapsulated nanoparticles（Cur-NPs）,which significantly improved the solubility,stability and cellular uptake of curcumin. Moreover,the Cur-NPs were superior to free curcumin in the matter of inhibition on the proliferation,migration and invasion of osteosarcoma 143B cells. It was found that both free curcumin and Cur-NPs could decrease the expressions of c-Myc and MMP7 in the level of mRNA and protein,which explained why free curcumin and Cur-NPs could inhibit the proliferation and invasion of metastatic osteosarcoma 143B cells. The Cur-NPs provided a promising strategy for metastatic osteosarcoma treatment.
骨肉瘤是一种高转移性的恶性肿瘤,5年生存率不到20%.姜黄素(Cur)具有治疗癌症的潜在功效,但其自身水溶性和稳定性差等性质限制了其临床使用.本文用聚乙二醇-聚(D,L-丙交酯-乙交酯)/聚(ε-己内酯)负载姜黄素形成纳米粒子,可以改善姜黄素的水溶性、稳定性和细胞内吞.与非负载的姜黄素相比,姜黄素纳米粒子在抑制骨肉瘤细胞增殖和迁移方面显示出明显的优势.研究结果表明,姜黄素和其纳米粒子均可降低c-Myc和MMP7表达,这可以很好地解释姜黄素纳米粒子抑制骨肉瘤细胞的机制.
作者
Guanyi Wang
Wantong Song
Na Shen
Haiyang Yu
Mingxiao Deng
Zhaohui Tang
Xueqi Fu
Xuesi Chen
王冠祎;宋万通;沈娜;于海洋;邓明虓;汤朝晖;付学奇;陈学思(Edmond H. Fischer Signal Transduction Laboratory, School of Life Sciences, Jilin University, Changchun 130012, China;Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;College of Chemistry, Northeast Normal University, Changchun 130024, China)
基金
supported by the National Natural Science Foundation of China (51520105004,51673189,51390484,51403204,51673185,51473029 and 51503202)
Science and Technology Service Network Initiative (KFJ-SW-STS-166)
the Chinese Academy of Sciences Youth Innovation Promotion Association
