乙型肝炎病毒DNA定量、分型检测与血清学检测的相关性分析

Hepatitis B virus DNA quantitative,classification test and serological test of correlation analysis

目的探讨乙型肝炎病毒血清学与DNA定量及基因分型检测间的相关性。方法选取68例乙肝血清学及DNA定量阳性的样本,使用ELISA法对乙肝血清学进行检测,荧光PCR法进行DNA定量及基因分型检测,分析血清学结果与DNA定量及基因分型结果的相关性。结果大三阳与小三阳样本HBV DNA定量结果之间存在显著性差异(P<0.01),而与HBV基因型无统计学差异(P>0.05),HBV DNA定量结果与基因型之间也无统计学差异(P>0.05)。HBV基因型在西安地区的分布与其他相关研究报道一致,以B、C型为主,且C型高于B型。结论HBV血清学结果与HBV DNA载量之间有密切的相关性,但与基因型分布无相关性,HBV DNA载量与基因型之间无相关性。

Objective To investigate the correlation between the hepatitis B virus serologic and HBV DNA and genotyping detection. Methods Sixty-eight samples of hepatitis B serology and HBV DNA positive samples were selected. HBV serology was detected by ELISA. DNA quantification and genotyping were performed by fluorescence PCR.The results of serological analysis were correlated with DNA quantification and genotyping sex. Results There were significant differences between the quantitative results of HBs Ag and HBe Ag （P〈0. 01）,while there was no significant difference between HBV DNA quantification and genotype （P〈0. 05）. The distribution of HBV genotype in Xi＇an area was consistent with other related studies,mainly B and C,and C was higher than that of type B （P〈0. 05）.Conclusion There is a close correlation between HBV serological results and HBV DNA loads,but has no correlation with genotype distribution,no correlation between HBV DNA loads and genotype.