智能手机联用的有机磷农残速测设备及芯片

Handhold,one-step,and rapid electrochemical biosensor platform with smartphone interface

常用的杀虫剂中,有机磷类农药是其中使用最广的一种,它会对人类健康和自然环境造成严重的影响。而要对食品和水中微量的有机磷类农药进行检测,需要发展快速、灵敏、便携的有机磷农药检测设备。本文提出了一种手持设备和丝网印刷电极(SPE)芯片,用于快速、准确、一步式检测有机磷农药残留。手持设备与智能手机通过蓝牙进行数据传输,利用三电极传感芯片与乙酰胆碱酯酶的抑制反应,实现对敌百虫农药的检出限为1ng/mL,检测时间小于5 min,单次检测成本低于1元。该平台也可以用于床边检测(POCT)以及其他目标的快速检测。

Organophosphorous pesticide（OP）,one of the most commonly used pesticides,could have serious adverse effects on human health and the environment.As the maximum residue limit（MRL）of OPs in food and water is low,it is in great demand to develop integrated,portable,user-friendly devices for the rapid and sensitive detection of OP residue.This work aims to present a homemade handhold device and screen printing electrode（SPE）chip for rapid,sensitive,and one-step detection of OPs.A handhold device was designed and fabricated to connect with smartphone to readout the electrochemical signals.Taking advantage of a three-electrode sensor chip and the inhibiting reaction of acetylcholine esterase（AchE）,Trichlorphon can be detected in a considerable low detection limit of 1ng/mL.By using the as-fabricated device and chip,the detection time was only 5min and each test costs less than $1.The platform will allow point-of-care testing（POCT）of OPs and other targets,which shows promise for healthy and environmental monitoring.