敦煌下瘀血方对SPID模型大鼠血清炎症细胞因子的影响

Effects of Dunhuang Xiayuxue Formula on the expression of inflammatory cytokines in serum of SPID rats

探讨敦煌下瘀血方对SPID模型大鼠血清IL-6、IL-8、TNF-α表达的影响。将50只SPF级雌性SD大鼠随机分为空白组、模型组及敦煌下瘀血方低剂量组、中剂量组、高剂量组,除空白组外均进行SPID造模。造模30 d后,空白组及模型组灌胃生理盐水,4 m L/只,敦煌下瘀血方高剂量组、中剂量组、低剂量组则分别灌服12.5倍、6.25倍、3.175倍敦煌下瘀血方,1次/d,共15 d。灌药结束后,除模型组外,其余各组大鼠体质量均较造模前有增加,与造模前相比较P<0.05或P<0.01;模型组与其余各组比较,体质量较低(P<0.01)。与空白组比较,其余各组大鼠血清IL-6、IL-8、TNF-α水平升高(P<0.01或P<0.05);与模型组相比较,各用药组大鼠血清IL-6、IL-8、TNF-α水平下降(P<0.01或P<0.05);各用药组间相比较,中剂量组、高剂量组大鼠血清IL-6、TNF-α水平较低剂量低(P<0.01或P<0.05),高剂量组大鼠血清IL-8与低剂量组、中剂量组相比较,P<0.01。结论:敦煌下瘀血方可降低SPID模型大鼠血清IL-6、IL-8、TNF-α水平。

To investigate the effect of Dunhuang Xiayuxue Formula on the expression of serum IL-6,IL-8 and TNF-α in SPID rats. Methods： 50 SPF female SD rats were randomly divided into blank group,model group,low-dose group,middle-dose and high-dose group,and SPID model was performed in addition to the blank group. 30 days later,the blank group and model group were treated with physiological saline 4 m L,while the high-dose group,middle-dose group and low-dose group was respectively given 12.5 times,6.25 times and 3.175 times Dunhuang Xiayuxue Formula once daily for 15 days. Results： Except the model group,the weight of rats in the other groups were all increased compared with before-modeling（P 0.05 or P 0.01）. And the model group was lower in weight compared to the other group（P 0.01）. Compared with the blank group,the level of serum IL-6,IL-8 and TNF-α of rats in the other groups were all increased（P 0.01 or P 0.05）;Compared with the model group,the level of serum IL-6,IL-8 and TNF-α in treatment groups were all decreased（P 0.01 or P 0.05）. In the middle-dose group and the high-dose group,the level of serum IL-6 and TNF-α were lower than those in the low-dose group（P 0.01 or P 0.05）. The serum IL-8 in the high-dose group was lower than that in the low-dose group and the middle-dose group（P 0.01）. Conclusion：Dunhuang Xiayuxue Formula can reduce the level of serum IL-6,IL-8 and TNF-α in rat SPID model.