副溶血性弧菌的耐药性及多位点序列分型

Antimicrobial resistance and multilocus sequencing typing of Vibrio parahemolyticus

目的了解近年来我国东部沿海地区某医院及周边海域的副溶血性弧菌分离株的耐药状况及多位点序列分型。方法对2012—2015年分离培养的40株副溶血性弧菌进行药敏试验;选取副溶血性弧菌的7对管家基因dna E、gyr B、rec A、dtd S、pnt A、pyr C和tna A作为靶标基因,对分离培养的40株副溶血性弧菌样本进行PCR扩增、测序及序列分析。结果菌株对氨苄西林的耐药率为100%,对头孢唑啉的中介率为37.5%,对头孢他啶、亚胺培南、左氧氟沙星等15种抗菌药则完全敏感。40株副溶血性弧菌有22个ST型,检测出17个新的ST型。结论多位点序列分型和药敏结果对研究副溶血性弧菌进化关系、食源性疾病的防控和临床合理用药提供了技术支持和数据参考。

Objective： To study the current status of multilocus sequencing typing（ MLST） and drug resistance in Vibrio parahaemolyticus（ VP） isolates from a hospital in the eastern coastal area of China and the surrounding waters. Methods：40 VP isolated from 2012 to 2015 were studied by the drug sensitive test; 7 house-keeping genes of VP（ dna E、gyr B、rec A、dtd S、pnt A、pyr C and tna A） were selected as the target genes,and the products of 40 VP isolated were amplified,sequenced and analyzed. Results： 22 sequences types（ ST） were identified among the 40 VP strains by using MLST analysis,17 new types were found. The resistant rate of the VP strains to ampicillin was 100%,and the mediation rate of the VP strains to cefazolin was 37. 5%,but no resistance to other 15 antibiotics,including ceftazidime,imipenem and levofloxacin were detected. Conclusion： MLST and drug susceptibility results would provide scientific support and data reference for the study on evolution,prevention and control of foodborne diseases and clinical rational drug use.