富氢水后处理对大鼠肾脏缺血再灌注后抗氧化损伤作用及机制研究

The effects and mechanism research of post-conditioning with rich hydrogen water against oxidation of renal ischemia-reperfusion injury in rats

目的探讨富氢水后处理对抗大鼠肾脏缺血再灌注(IR)后氧化应激损伤的作用及其机制。方法选择健康雄性SD大鼠30只,质量230~250 g,釆用随机数字表法分为3组(n=10),假手术组(Sham组)、缺血再灌注组(IR组)和富氢水组(HS组)。Sham组:腹正中切口开腹,游离两侧肾脏,分离并结扎右侧肾蒂,分离左侧肾蒂不予结扎,暴露手术野30 min后缝合;IR组:分离左侧肾蒂并用动脉夹阻断血流,30 min后恢复血液灌注,制备大鼠肾脏缺血再灌注模型;HS组:前期处理同IR组,再灌前即刻给与2%富氢水(10 ml·kg-1·d-1)灌肠。其余两组同法给与同等剂量生理盐水。造模成功后,各组分别于再灌后3 h、6 h、12 h、24 h采取血清标本,测定各组大鼠血尿素氮(BUN)、血清肌酐(SCr)、血清超氧化物歧化酶(SOD)、血清丙二醛(MDA)。结果各组大鼠BUN和SCr水平与Sham组比较均明显升高(P<0.05)。IR组BUN和SCr水平随时间延长而逐渐升高,组间差异有统计学意义(P<0.05)。与IR组比较,HS组BUN和SCr各时间段水平均降低(P<0.05)。SOD和MDA结果显示:各组与Sham组比较,IR组大鼠SOD水平均明显降低,MDA水平均明显升高,差别有统计学意义(P<0.05)。与IR组比较,HS组SOD水平升高,MDA水平降低,差别有统计学意义(P<0.05)。结论富氢水后处理对大鼠肾脏IRI可产生保护作用,作用机制可能是通过选择性抗氧化、抑制炎症因子释放来实现。

Objective： To investigate the effects of hydrogen-rich water treatment on post-oxidative stress injury in rats with renal ischemia reperfusion（ IR） and its mechanism. Methods： 30 healthy male SD rats,the quality of 230 ~ 250 g. With random number table method was divided into 3 groups（ n = 10）,control group（ Sham）,ischemia reperfusion group（ IR group） and hydrogen rich water group（ HS）. The ventral median incision in Sham group was opened,and the kidneys were dissociated,separated and ligated with the right kidney. The left kidney was not ligated,and the surgical field was closed after 30 min. The renal ischemia-reperfusion model of rat kidney was prepared after 30 min of blood perfusion. The HS group was treated with the IR group and immediately gave 2% hydrogen rich water（ 10 ml·kg-1·d-1） enema before irrigation. The other two groups were given the same dose of saline. After the success of the building,each group respectively in reperfusion after 3 h,6 h,12 h,24 h to serum specimens,the determination of each group rats blood urea nitrogen（ BUN）,serum creatinine（ SCr）,serum superoxide dismutase（ SOD）,serum malondialdehyde（ MDA）. Results： Both the BUN and SCr levels of rats in each group were significantly higher than those in Sham group（ P〈0. 05）. The level of BUN and SCr of IR group increased gradually with time,and the difference between groups was statistically significant（ P〈0. 05）. Compared with IR group,the levels of the HS group BUN and SCr were reduced at each time period（ P〈0. 05）. The results of SOD and MDA showed that the SOD levels of the rats in IR group were significantly lower than those in the Sham group,and the MDA level was significantly higher,and the difference was statistically significant（ P〈0. 05）. Compared with IR group,SOD level of HS group increased,MDA level decreased,and the difference was statistically significant（ P〈0. 05）. Conclusion： the treatment of IRI in rats can be protected by hydrogen-rich water treatment,which may be achieved by selective antioxidation and inhibition of inflammatory cytokines.