神阙灸法治疗EAT模型小鼠的疗效及机制初探

Effect and possible mechanism of Shenque (CV8) moxibustion therapy on the mice with autoimmune thyroiditis

目的:观察神阙灸法对实验性自身免疫性甲状腺炎(EAT)模型小鼠的疗效并探讨可能机制。方法:运用猪甲状腺球蛋白(PTg)皮下多点免疫联合饮用高碘水的方法制作小鼠EAT模型,并随机分为空白组、模型组、治疗组。采用酶联免疫吸附法(ELISA)检测小鼠促甲状腺素(TSH)、甲状腺球蛋白抗体(TGAb)水平;苏木素-伊红染色(HE)观察甲状腺及神阙穴局部皮肤组织形态;免疫组织化学法检测甲状腺、神阙穴局部皮肤组织中的转化生长因子-β1(TGF-β1)、瞬时受体电位香草酸亚家族-1(TRPV1)水平。结果:与模型组相比,治疗组小鼠甲状腺组织充血、水肿、滤泡上皮变性坏死和炎症细胞浸润均较轻,甲状腺球蛋白合成正常,且小鼠血清TGAb水平显著下降(P<0.05),神阙穴局部皮肤组织TGF-β1、TRPV1表达增强(P<0.05),而血清TSH、甲状腺组织TGF-β1、TRPV1水平未见显著差异(P>0.05)。结论:神阙灸法能够显著减轻EAT小鼠甲状腺组织炎症反应,其疗效可能与神阙灸法激活局部组织TGF-β1、TRPV1表达,继而抑制免疫反应,使EAT小鼠甲状腺自身抗体水平下降有关。

Objective： To investigate the effect and possible mechanism of Shenque（CV8） moxibustion therapy on the mice with autoimmune thyroiditis.Methods： Experimental autoimmune thyroiditis（EAT） model was replicated on mice with high iodine intake and subcutaneous immunization of porcine thyroglobulin（PTg）.The mice were randomly divided into blank group,EAT model group and treatment group.The thyroid stimulating hormone（TSH）concentrations and thyroglobulin antibody（TGAb） concentrations were detected by enzyme-linked immunosorbent assay（ELISA）.The morphological changes of thyroids and local skin tissues around CV8 were observed by HE staining.The expression of cytokine transforming growth factor-β1（TGF-β1） and transient receptor potential vanilloid subfamily member 1（TRPV1） of thyroid tissues and local skin tissues around CV8 were detected by immunohistochemistry（IHC）.Results： Comparing with the EAT model group,the mice from treatment group presented lighter congestion,swollen and inflammatory cells infiltration,degeneration and necrosis of follicular epithelial cells in thyroid tissues,along with normal production of thyroglobulin.The expression of TGF-β1 and TRPV1 in the local skin tissues around CV8 were enhanced significantly after treatment（P〈0.05）,however,there were no significant changes in the thyroid tissues（P〈0.05）.The serum TGAb concentrations of treatment group were significantly lower comparing with the EAT model group（P〈0.05）,however,there was no significant differences in the serum TSH concentrations among groups（P〉0.05）.Conclusion： Using Moxibustion therapy on CV8 can significantly ease the inflammation in the thyroid tissues of EAT mice.The up regulation of TGF-β1 and TRPV1 in the local skin tissues around CV8 after moxibustion therapy may suppress the immune response,moreover,causing a down regulation of thyroid autoantibody.