CNPs对肾结石大鼠尿液NGAL、MCP-1水平和肾功能的影响

Analysis of NGAL and MCP-1 levels in rats with kidney stones after CNPs modeling

目的:探讨钙化性纳米微粒(CNPs)建立大鼠肾结石模型对其尿液中中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、单核细胞趋化蛋白-1(MCP-1)水平和肾功能的影响。方法:选取SPF级雄性SD大鼠24只,采用随机数字表法分为实验组、空白组各12只,实验组采取静脉注射CNPs建造大鼠肾结石模型,空白组注射等量生理盐水,检测实验后不同时间点两组大鼠尿液中NGAL、MCP-1的水平,结石形成情况,大鼠肾脏病理变化及肾功能变化。结果:实验组大鼠在鼠造模后4 h、12 h、24 h、1周、2周、8周尿液中NGAL、MCP-1水平呈现逐渐升高的趋势,并且每一个时间点对应的尿液NGAL水平均高于空白组(P<0.05),造模后第1周、2周、8周时对应的尿液MCP-1水平均高于空白组(P<0.05);实验组大鼠尿素氮、血肌酐水平在造模后第8周均高于空白组(P<0.05);实验组大鼠肾脏病理检查均发现结石结晶形成,空白组未发现结石结晶形成。结论:CNPs建立大鼠肾结石模型的作用机制可能与升高NGAL、MCP-1水平有关,CNPs可造成肾脏功能的损伤。

Objective： To investigate the effects of the calcifying nanoparticles（CNPs） to establish a rat model of kidney stones on the levels of urine neutrophil gelatinase associated lipid carrier pale（NGAL）,white light monocyte chemoattractant protein-1（MCP-1） and kidney function.Methods： 24 male SD SPF rats were randomly divided into experimental group and control group with 12 rats in each group,the experimental group adopted intravenous injection of CNPs to build rat renal calculus model,the control group was injected with saline.The level of MCP-1 and NGAL in rat urine,stone formation in rat kidney,pathological changes and the changes of renal function were measured at different time points.Results： The urine NGAL and MCP-1 levels in the experimental group after modeling 4 h,12 h,24 h,1 week,2 weeks and 8 weeks showed increasing tendency,and the urine NGAL levels at each time point were higher than that in control group（P〈0.05）,after modeling 1 week,2 weeks and 8 weeks the urine MCP-1 levels were higher than those of control group（P〈0.05）.The level of serum creatinine,urea nitrogen in rats of the experimental group in 8 weeks after surgery were higher than that in control group（P〈0.05）.Renal biopsy were found in the formation of stone crystals in the experimental group,the control group did not find the crystal stones form.Conclusion： The mechanism of CNPs to establish rat kidney stones model may be related to the increase of NGAL and MCP-1 levels,and CNPs can cause kidney functional damage.