丹参酮IIA对成年大鼠睾丸扭转的保护作用和机制研究

The mechanism research for Protective effect of tanshinone ⅡA on testicular torsion in adult rats

目的:观察丹参酮IIA对成年大鼠睾丸扭转复位睾丸损伤的保护作用并初步探讨其机制。方法:对建立左侧睾丸扭转复位动物模型,将36只7周龄健康SD雄性大鼠随机分为3组:假手术组、生理盐水组、丹参酮IIA组,每组12只;分别于术后72小时、4周两批处死大鼠,每批每组6只,取左侧睾丸,行HE染色及组织损伤评分,Tunel染色,以及睾丸组织内Bcl-2蛋白检测(免疫印迹法),检测总活性氧(ROS),超氧化物歧化酶(SOD)、与丙二醛(MDA)含量,并测定大鼠血清睾酮水平。结果:与生理盐水组比较,丹参酮II给药组在术后72小时睾丸组织损伤明显减轻,细胞凋亡减少,Bcl-2表达升高;在术后4周发现给药组SOD、大鼠血清睾酮水平明显上升,而组织内ROS和MDA水平含量下降,差异均具有显著性(P<0.05)。结论:单次腹腔内注射丹参酮IIA对成年大鼠单侧睾丸扭转损伤具有保护作用,其机制在早期可能是通过抗凋亡作用,而在远期则可能发挥了抗氧化作用。

Objective： To observe the protective effect of tanshinone IIA on testicular torsion and return to testicular injury in adult rats and to explore its mechanism.Methods： 36 male SD rats aged 7 weeks were randomly divided into 3 groups： sham operation group,saline group and tanshinone IIA group,12 rats in each group.The rats were sacrificed at 72 hours and 4 weeks after operation.Six rats in each group and left testes were subjected to HE staining and histological injury score,Tunel staining,and Bcl-2 protein test（immunoblotting）,reactive oxygen（ROS）,superoxide dismutase（SOD） and malondialdehyde（MDA） were measured.Serum testosterone levels were measured as well.Results： Compared with the saline group,the testis tissue injury was significantly reduced in the tanshinone IIA group,the apoptotic cells were decreased and the expression of Bcl-2 was increased.The levels of SOD and the level of serum testosterone in rats was significantly higher than that in saline group（P 0.05）.Conclusion： Single intraperitoneal injection of tanshinone II has protective effect on unilateral testicular torsion injury in adult rats,possibly through the anti-apoptotic effect in early stage,and may play an antioxidant role in the long term.